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Nonthermal Atmospheric Argon Plasma Jet Effects on Escherichia coli Biomacromolecules

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Abstract

Nonthermal atmospheric plasma jet, a promising technology based on ionized gas at low temperatures, can be applied for disinfection of contaminated surfaces. In this study, Escherichia coli cells and their macromolecules were exposed to the nonthermal atmospheric argon plasma jet for different time durations. Total protein, genomic DNA, and malondialdehyde (MDA) levels of E. coli were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining; agarose gel electrophoresis; and measurement of absorbance at 534 nm, respectively. After exposure, the spectroscopic results of liquid samples indicated that the survival reduction of E. coli can reach to 100 % in an exposure time of 600 s. Moreover, inactivation zones of E. coli, DNA degradation, and MDA levels were significantly increased. Additionally, banding patterns of total protein were changed and amino acid concentrations increased following ninhydrin test. The experimental results suggest that the nonthermal plasma could serve as an effective instrument for both sterilizing E. coli and degrading macromolecules from the surface of the objects being sterilized.

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Abbreviations

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

ROS:

Reactive oxygen species

UV:

Ultraviolet

LB:

Luria–Bertani

DTT:

Dithiothreitol

MDA:

Malondialdehyde

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Acknowledgments

This investigation was supported by a biotechnology grant (ID: 33/D/379) from the University of Mazandaran Research and Technology Deputy.

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Authors and Affiliations

  1. Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, 47416-95447, Mazandaran, Iran

    Abasalt Hosseinzadeh Colagar & Hamed Memariani

  2. Department of Atomic and Molecular Physics, Faculty of Basic Sciences, University of Mazandaran, Babolsar, 47416-95447, Mazandaran, Iran

    Farshad Sohbatzadeh & Azadeh Valinataj Omran

  3. Nano and Biotechnology Research Group, Faculty of Basic Sciences, University of Mazandaran, Babolsar, 47416-95447, Mazandaran, Iran

    Abasalt Hosseinzadeh Colagar & Farshad Sohbatzadeh

Authors
  1. Abasalt Hosseinzadeh Colagar
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  2. Hamed Memariani
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  3. Farshad Sohbatzadeh
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  4. Azadeh Valinataj Omran
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Correspondence to Abasalt Hosseinzadeh Colagar.

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Hosseinzadeh Colagar, A., Memariani, H., Sohbatzadeh, F. et al. Nonthermal Atmospheric Argon Plasma Jet Effects on Escherichia coli Biomacromolecules. Appl Biochem Biotechnol 171, 1617–1629 (2013). https://doi.org/10.1007/s12010-013-0430-9

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