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Non-Selective Toxicological Effects of the Insect Juvenile Hormone Analogue Methoprene. A Membrane Biophysical Approach

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Abstract

The Gram-positive bacterium, Bacillus stearothermophilus, was used as a model organism to identify the non-selective toxic effects of the currently used insecticide methoprene (isopropyl(2E,4E)-11-methoxy-3,7,11-trimethyl-2,4-dodecadienoate). A significant decrease of the yield of bacterial cultures and a premature appearance of ultrastructural abnormalities in cells cultured in the presence of the insecticide were taken as indicators of cytotoxicity. A putative correlation of this cytotoxicity with methoprene-induced perturbations on membrane lipid organization was investigated, using differential scanning calorimetry and the fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its propionic acid derivative (DPH–PA). The membrane physical effects depended on the lipid bilayer composition and packing. The most striking effect was a progressive broadening and shifting to lower temperatures, with increasing methoprene concentrations, of the main transition phase of the dimyristoyl- or dipalmitoylphosphatidylcholine bilayers and of the lateral phase separation of liposomes reconstituted with the lipid extracts of B. stearothermophilus.

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Abbreviations

DMPC:

dimyristoylphosphatidylcholine

DPH:

1,6-diphenyl-1,3,5-hexatriene

DPH-PA:

3-(p-(6-phenyl)-1,3,5-hexatrienyl)phenylpropionic acid

DPPC:

dipalmitoylphosphatidylcholine

DSC:

differential scanning calorimetry

TEM:

transmission electron microscopy

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Authors and Affiliations

  1. Centro de Neurociências e Biologia Celular; Department of Biochemistry, University of Coimbra, 3001-401, Coimbra, Portugal

    João P. Monteiro, Manuel J. Matos & Amália S. Jurado

  2. Higher School of Technology, Polytechnic Institute of Viseu, 3504-510, Viseu, Portugal

    Romeu A. Videira

  3. Laboratório de Microscopia Electrónica, Department of Botany, University of Coimbra, 3001-401, Coimbra, Portugal

    Augusto M. Dinis

  4. Department of Biochemistry, University of Coimbra, Apartado 3126, 3001-401, Coimbra, Portugal

    Amália S. Jurado

Authors
  1. João P. Monteiro
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  2. Romeu A. Videira
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  3. Manuel J. Matos
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  4. Augusto M. Dinis
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  5. Amália S. Jurado
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Correspondence to Amália S. Jurado.

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Monteiro, J.P., Videira, R.A., Matos, M.J. et al. Non-Selective Toxicological Effects of the Insect Juvenile Hormone Analogue Methoprene. A Membrane Biophysical Approach. Appl Biochem Biotechnol 150, 243–257 (2008). https://doi.org/10.1007/s12010-007-8127-6

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  • DOI: https://doi.org/10.1007/s12010-007-8127-6

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