Abstract
Very-long-chain fatty acids (VLCFAs) are important precursors of many cellular hydrophobic substances involved in many aspects of plant growth and development. They are synthesized by a microsomal fatty acid elongation (FAE) system through sequential additions of C2 moiety to long-chain fatty acid. While the FAE process has been intensively investigated, its regulatory mechanism remains to be elucidated. Here, we report that the R2R3-type MYB96 transcription factor regulates fatty acid elongation in seeds by directly modulating expression of FAE1, an well-known seed-specific β-ketoacyl–CoA synthase (KCS) that catalyzes the first condensation reaction in VLCFA elongation. The FAE1 gene was up-regulated in MYB96-overexpressing activation-tagging myb96-ox seeds, but down-regulated in MYB96-deficient myb96-1 seeds. Consistently, accumulation of C20 and C22 fatty acids was significantly elevated in myb96-ox seeds, whereas their accumulation was reduced in myb96-1 seeds. These findings suggest that MYB96 directly regulates FAE1 to stimulate C20 accumulation possibly in the regulation of seed maturation and development.
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Acknowledgments
This work was supported by the Basic Science Research (NRF-2013R1A1A1004831) and Global Research Network (NRF-2014S1A2A2028392) programs provided by the National Research Foundation of Korea and by the Cooperative Research Program for Agriculture Science & Technology Development (SSAC, Project No. PJ01108101) provided by the Rural Development Administration (H.U.K). H.G.L. was supported by the BK21 PLUS program in the Department of Bioactive Material Sciences.
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Pil Joon Seo and Hyun Uk Kim have contributed equally to this paper.
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Lee, H.G., Park, BY., Kim, H.U. et al. MYB96 stimulates C18 fatty acid elongation in Arabidopsis seeds. Plant Biotechnol Rep 9, 161–166 (2015). https://doi.org/10.1007/s11816-015-0352-9
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DOI: https://doi.org/10.1007/s11816-015-0352-9