Skip to main content
SpringerLink
Log in

Antifungal Activity of Leaf Essential Oil and Extracts of Metasequoia glyptostroboides Miki ex Hu

  • Original Paper
  • Published:
Journal of the American Oil Chemists' Society

Abstract

Plant diseases constitute an emerging threat to global food security. Many of the currently available antifungal agents for agriculture are highly toxic and nonbiodegradable and cause extensive environmental pollution. Moreover, an increasing number of phytopathogens are developing resistance to them. Therefore, the aim of this study was to assess the antifungal efficacy of the leaf essential oil and the leaf extracts of Metasequoia glyptostroboides against Fusarium oxysporum, Fusarium solani, Phytophthora capsici, Colletotrichum capsici, Sclerotinia sclerotiorum, Botrytis cinerea and Rhizoctonia solani. The oil (1,000 μg/disc) and the extracts (1,500 μg/disc) revealed a remarkable antifungal effect against the tested plant pathogenic fungi with a radial growth inhibition percentage of 41.3–66.3% and 13.4–54.4%, respectively along with their respective MIC values ranging from 62.5 to 1,000 μg/ml and 500–4,000 μg/ml. The oil had a strong detrimental effect on spore germination of all the tested plant pathogens along with the concentration as well as time-dependent kinetic inhibition of Botrytis cinerea. Also, the oil exhibited a potent in vivo antifungal effect against Phytophthora capsici on greenhouse grown pepper plants. The results of this study indicate that the oil and extracts of M. glyptostroboides leaves could become natural alternatives to synthetic fungicides to control certain important plant fungal diseases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Montesinos E (2007) Antimicrobial peptides and plant disease control. FEMS Microbiol Lett 270:1–11

    Article  CAS  Google Scholar 

  2. Savary S, Teng PS, Willocquet L, Nutter FW (2006) Quantification and modeling of crop losses: a review of purposes. Annu Rev Phytopathol 44:89–112

    Article  CAS  Google Scholar 

  3. Paster N, Bullerman LB (1988) Mould spoilage and mycotoxin formation in grains as controlled by physical means. Int J Food Microbiol 7:257–265

    Article  CAS  Google Scholar 

  4. Knight SC, Anthony VM, Brady AM, Greenland AJ, Heaney SP, Murray DC, Powell KA, Schulz MA, Spinks CA, Worthington PA, Youle D (1997) Rationale and perspectives on the development of fungicides. Annu Rev Phytopathol 35:349–372

    Article  CAS  Google Scholar 

  5. Brent KJ, Hollomon DW (1998) Fungicide resistance: the assessment of risk. FRAC, Global Crop Protection Federation, Brussels, pp 1–48

    Google Scholar 

  6. Barnard M, Padgitt M, Uri ND (1997) Pesticides use and its measurement. Inter Pest Control 39:161–164

    Google Scholar 

  7. Daoubi M, Hernandez-Galan R, Benharref A, Collado IG (2005) Screening study of lead compounds for natural product-based fungicides: antifungal activity and biotransformation of 6-alpha, 7-alpha-dihydroxy-betahimachalene by Botrytis cinerea. J Agric Food Chem 53:6673–6677

    Article  CAS  Google Scholar 

  8. Walsh TJ, Viviani MA, Arathoon E, Chiou C, Ghannoum M, Groll AH, Odds FC (2000) New targets and delivery systems for antifungal therapy. Med Mycol 38(1):335–347

    CAS  Google Scholar 

  9. Barker KS, Rogers PD (2006) Recent insights into the mechanisms of antifungal resistance. Curr Infect Dis Rep 8:449–456

    Article  Google Scholar 

  10. Duke SO (1990) Natural pesticides from plants. In: Janick J, Simon JE (eds) Advances in new crops. Timber Press, Portland, pp 511–517

    Google Scholar 

  11. Costa TR, Fernandes FLF, Santos SC, Oliveria CMA, Liao LM, Ferri PH, Paulo JR, Ferreira HD, Sales BHN, Silva MRR (2000) Antifungal activity of volatile constituents of Eugenia dysenterica leaf oil. J Ethnopharmcol 72(2):111–117

    Article  CAS  Google Scholar 

  12. Negi PS, Chauhan AS, Sadia GA, Rohinishree YS, Ramteke RS (2005) Antioxidant and antibacterial activities of various seabuckthorn (Hippophae rhamnoides L.) seed extracts. Food Chem 92:119–124

    Article  CAS  Google Scholar 

  13. Bajpai VK, Kang SC (2009) Potential role of leaf essential oil and extracts of Metasequoia glyptostroboides Miki ex Hu to inhibit the growth of Listeria monocytogenes spp. J Food Biochem (in press)

  14. Duru ME, Cakir A, Kordali S, Zengin H, Harmandar M, Izumi S, Hirata T (2003) Chemical composition and antifungal properties of essential oils of three Pistacia species. Fitoterapia 74:170–176

    Article  CAS  Google Scholar 

  15. Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolke RH (1995) Manual of clinical microbiology vol 6. ASM, Washington

    Google Scholar 

  16. Leelasuphakul W, Hemmanee P, Chuenchitt S (2008) Growth inhibitory properties of Bacillus subtilis strains and their metabolites against the green mold pathogen (Penicillium digitatum Sacc.) of citrus fruit. Postharvest Biol Tech 48(1):113–121

    Article  CAS  Google Scholar 

  17. Rana BK, Singh UP, Taneja V (1997) Antifungal activity and kinetics of inhibition by essential oil isolated from leaves of Aegle marmelos. J Ethanopharmacol 57:29–34

    Article  CAS  Google Scholar 

  18. Lee SE, Park BS, Kim MK, Choi WS, Kim HT, Cho KY, Lee SG, Lee HS (2001) Antifungal activity of pipernonaline, a piperidine alkaloid derived from long pepper. Piper longum L., against phytopathogenic fungi. Crop Prot 20:523–528

    Article  CAS  Google Scholar 

  19. Pandey DK, Tripathi NN, Tripathi RD, Dixit SN (1982) Fungitoxic and phytotoxic properties of the essential oil Caesulia axillaris Roxb. Angewan Bot 56:259–267

    Google Scholar 

  20. Morris JA, Khettry A, Seitz EW (1979) Antimicrobial activity of aroma chemicals and essential oils. J Am Oil Chem Soc 56:595–603

    Article  CAS  Google Scholar 

  21. Bajpai VK, Rahman A, Kang SC (2007) Chemical composition and antifungal properties of the essential oil and crude extracts of Metasequoia glyptostroboides Miki ex Hu. Ind Crop Prod 26:28–35

    Article  CAS  Google Scholar 

  22. Sidhu OP, Chandra H, Behl HM (2009) Occurrence of aflatoxins in mahua (Madhuca indica Gmel.) seeds: synergistic effect of plant extracts on inhibition of Aspergillus flavus growth and aflatoxin production. Food Chem Toxicol 47:774–777

    Article  CAS  Google Scholar 

  23. Gudzic B, Djokovic D, Vajs V, Palic R, Stojanovic G (2002) Composition and antimicrobial activity of the essential oil of Hypericum maculatum Crantz. Flavour Frag J 17(5):392–394

    Article  CAS  Google Scholar 

  24. Cakir A, Kordali S, Zengin H, Izumi S, Hirata T (2004) Composition and antifungal activity of essential oils isolated from Hypericum hyssopifolium and Hypericum heterophyllum. Flavour Frag J 19(1):62–68

    Article  CAS  Google Scholar 

  25. Bajpai VK, Kim HR, Hou CT, Kang SC (2009) Microbial conversion and in vitro and in vivo antifungal assessment of bioconverted docosahexaenoic acid (bDHA) used against agricultural plant pathogenic fungi. J Ind Microbiol Biotechnol 36(5):695–704

    Article  CAS  Google Scholar 

  26. Yoo JK, Ryu KH, Kwon JH, Ahn YJ (1998) Antifungal activities of oriental medicinal plant extracts against phytopathogenic fungi. Korean J Agric Chem Biotechnol 41:600–604

    Google Scholar 

  27. Lee SO, Choi GJ, Jang KS, Lim HK, Cho KY, Kim JC (2007) Antifungal activity of five plant essential oils as fumigant against postharvest and soilborne plant pathogenic fungi. Plant Pathol J 23(2):97–102

    Google Scholar 

  28. Alvarez-Castellanos PP, Bishop CD, Pascual-Villalobos MJ (2001) Phytochemistry 57:99–102

    Google Scholar 

  29. Singh G, Singh OP, Maurya S (2002) Program crystal growth and characterization. 45:75–81

  30. Bouchra C, Achouri M, Hassani LMI, Hmamouchi M (2003) Chemical composition and antifungal activity of essential oils of seven Moroccan Labiatae against Botrytis cinerea Pers. J Ethnopharmacol 89:165–169

    Article  CAS  Google Scholar 

  31. Elad Y (1991) Multiple resistance to benzimidazoles dicarboximides and diethofencarb in field isolates of Botrytis cinerea in Israel. Plant Pathol 41:41–46

    Article  Google Scholar 

  32. Marino M, Bersani C, Comi G (2001) Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceace and Compositae. Int J Food Microbiol 67:187–195

    Article  CAS  Google Scholar 

  33. Tuley de Silva K (1996) A manual on the essential oil industry. United Nations Industrial Development Organization, Vienna

    Google Scholar 

Download references

Acknowledgment

This research was supported by Daegu University Research Grant, 2009.

Author information

Authors and Affiliations

  1. Department of Biotechnology, College of Engineering, Daegu University, Kyoungsan, Kyoungbook, 712-714, Republic of Korea

    Vivek K. Bajpai & Sun Chul Kang

Authors
  1. Vivek K. Bajpai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sun Chul Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sun Chul Kang.

About this article

Cite this article

Bajpai, V.K., Kang, S.C. Antifungal Activity of Leaf Essential Oil and Extracts of Metasequoia glyptostroboides Miki ex Hu. J Am Oil Chem Soc 87, 327–336 (2010). https://doi.org/10.1007/s11746-009-1500-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11746-009-1500-6

Keywords

Search

Navigation