Lipids

, 44:345

Influence of Commercial Dietary Oils on Lipid Composition and Testosterone Production in Interstitial Cells Isolated from Rat Testis

  • Graciela E. Hurtado de Catalfo
  • María J. T. de Alaniz
  • Carlos Alberto Marra
Original Article

DOI: 10.1007/s11745-008-3277-z

Cite this article as:
de Catalfo, G.E.H., de Alaniz, M.J.T. & Marra, C.A. Lipids (2009) 44: 345. doi:10.1007/s11745-008-3277-z

Abstract

The aim of this study was to examine the influence of dietary fat on lipid composition, as well as on the steroidogenic function of interstitial cells isolated from Wistar rats that had been fed semi-synthetic diets containing four different commercial oils (S soybean, O olive, C coconut, and G grape seed). Steroidogenic enzyme activities, lipid composition, and androgen production were measured in testicular interstitial cells. Lipid analysis included measurement of the contents of major lipid subclasses (neutral lipids, polar lipids, free and esterified cholesterol), as well as principal polar and neutral lipid fatty acyl compositions. Significant differences in lipid composition were observed among the groups, most of them reflecting the specific fatty acyl composition of the diet tested. Testosterone concentration was higher in O and C groups compared with S or G. In agreement with this observation, the activity of both key enzymes involved in testosterone biosynthesis (3-β-HSD and 17-β-HSD) was higher in O and C groups with significant differences between them (O > C). A significant negative correlation was found between cellular testosterone production and cellular cholesterol ester content. Additionally, testosterone concentration directly correlated with cholesterol levels. We conclude that dietary oils qualitatively and quantitatively modified the lipid composition of interstitial cells, producing either a direct or indirect regulatory effect on testicular steroidogenic function.

Keywords

Lipid compositionFatty acidsTestosterone productionTesticular interstitial cellsDietary lipids

Abbreviations

C

Coconut

FC

Free cholesterol

CE

Esterified cholesterol

DBI

Double bond index

FA

Fatty acids

FAME

Fatty acid methyl esters

G

Grape seed

3β-HSD

3β-Hydroxysteroid dehydrogenase

17β-HSD

17β-Hydroxysteroid dehydrogenase

HPLC

High performance liquid chromatography

LH

Luteinizing hormone

MUFA

Monounsaturated FA

NL

Neutral lipids

O

Olive

PL

Polar lipids (phospholipids)

PUFA

Polyunsaturated FA

Rf

Relative chromatographic mobility

RIA

Radioimmunoassay

S

Soybean

SFA

Saturated FA

T

Testosterone

TL

Total lipids

TLC

Thin layer chromatography

TAG

Triacylglycerides

Copyright information

© AOCS 2008

Authors and Affiliations

  • Graciela E. Hurtado de Catalfo
    • 1
  • María J. T. de Alaniz
    • 1
  • Carlos Alberto Marra
    • 1
  1. 1.Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT-La Plata-CONICET-UNLP, Cátedra de Bioquímica, Facultad de Ciencias MédicasUniversidad Nacional de La PlataLa PlataArgentina