Lipids

, Volume 44, Issue 2, pp 97–102

The Depressive Effects of 5,8,11-Eicosatrienoic Acid (20:3n-9) on Osteoblasts

Authors

    • Department of Clinical Sciences, Institute of Natural MedicineUniversity of Toyama
  • Nobuo Suzuki
    • Noto Marine Laboratory, Institute of Nature and Environmental TechnologyKanazawa University
  • Retno Widyowati
    • Department of Natural Products Chemistry, Institute of Natural MedicineUniversity of Toyama
  • Tatsuro Miyahara
    • Department of Human Science, Faculty of MedicineUniversity of Toyama
  • Shigetoshi Kadota
    • Department of Natural Products Chemistry, Institute of Natural MedicineUniversity of Toyama
  • Hiroshi Ochiai
    • Department of Human Science, Faculty of MedicineUniversity of Toyama
  • Kei Hamazaki
    • Department of Clinical Sciences, Institute of Natural MedicineUniversity of Toyama
Original Article

DOI: 10.1007/s11745-008-3252-8

Cite this article as:
Hamazaki, T., Suzuki, N., Widyowati, R. et al. Lipids (2009) 44: 97. doi:10.1007/s11745-008-3252-8

Abstract

In cases of essential fatty acid deficiency, 5,8,11-eicosatrienoic acid (Mead acid, 20:3n-9) is synthesized from oleic acid as a 20-carbon analog of arachidonic acid. It was reported that 20:3n-9 levels were markedly higher in human fetal cartilage than in the muscle, liver and spleen. We, therefore, hypothesized that 20:3n-9 decreased osteoblastic activity. Goldfish scales were incubated either with 20:3n-9 or with oleic acid at 15 °C for 6 and 18 h. Both osteoblastic and osteoclastic activities in the scale were assessed by measuring alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase, respectively. MC3T3-E1 cells (an osteoblast cell line derived from the mouse) were incubated with 20:3n-9 or oleic acid at 37 °C for 6 and 18 h. ALP activity in cell lysate was measured. In the case of experiments with scales, 20:3n-9 (1–100 μM) significantly suppressed osteoblastic activity after 6 and 18 h of incubation, whereas oleic acid did not change this activity. Osteoclastic activity was not affected either by 20:3n-9 or by oleic acid. In the case with the cell line, osteoblastic activity was again significantly decreased with 20:3n-9 (10–30 μM) after 6-h incubation but not after 18 h incubation. The presence of 20:3n-9 in fetal cartilage may be important for the prevention of calcification in the cartilage. 20:3n-9 could be applied to some clinical situations where bone formation should be inhibited.

Keywords

BoneCartilageCo-cultureFatty acidsGoldfish scalesOsteoclasts

Abbreviations

20:3n-9

5,8,11-Eicosatrienoic acid (20:3n-9, Mead acid)

AA

Arachidonic acid

ALP

Alkaline phosphatase

TRAP

Tartrate-resistant acid phosphatase

CF

Cystic fibrosis

RANK

Receptor activator of NF-κB

RANKL

Receptor activator of NF-κB ligand

LTB4

Leukotriene B4

Copyright information

© AOCS 2008