Abstract
Seedlings of Camellia sinensis (L.) were grown hydroponically to study the effect of aluminium (Al) on leaf antioxidant defence system and cell ultrastructure. We found that malondialdehyde (MDA) content decreased at 0–0.32 mM Al, but increased significantly at 0.53 mM Al. Like MDA, hydrogen peroxide (H2O2) content increased at 0.53 mM Al; however, no differences were observed at 0–0.32 mM Al. Superoxide dismutase (SOD, EC1.15.1.1) activity remained practically constant at 0–0.32 mM Al, but increased sharply at 0.53 mM Al; catalase (CAT, EC1.11.1.6) and guaiacol peroxidase (GPX, EC1.11.1.7) activities decreased following an initial increase, reaching their peaks at 0.32 mM Al. Ascorbate peroxidase (APX, EC 1.11.1.11) activity increased and glutathione (GR, EC 1.6.4.2) level fluctuated with increasing Al concentrations. Transmission electron microscope analysis of Al-treated leaves showed that although cell ultrastructural integrity was maintained at 0–0.32 mM, significant membrane damage was observed at 0.53 mM. Our results suggest that at low Al concentrations, the leaf antioxidant defence system can scavenge reactive oxygen species and sufficiently protect cells from free radical injury. However, at higher Al concentrations (0.53 mM), the balance between formation and detoxification of ROS is lost, resulting in the destruction of cell ultrastructure.
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Abbreviations
- Al:
-
Aluminium
- APX:
-
Ascorbate peroxidase
- CAT:
-
Catalase
- GPX:
-
Guaiacol peroxidase
- GR:
-
Glutathione reductase
- H2O2 :
-
Hydrogen peroxide
- MDA:
-
Malondialdehyde
- ROS:
-
Reactive oxygen species
- SOD:
-
Superoxide dismutase
- TEM:
-
Transmission electron microscope
References
Ali B, Hasan S, Hayat S, Hayat Q, Yadav S, Fariduddin Q, Ahmad A (2008) A role for brassinosteroids in the amelioration of aluminium stress through antioxidant system in mung bean (Vigna radiata L. Wilczek). Environ Exp Bot 62:153–159. doi:10.1016/j.envexpbot.2007.07.014
Asthir B, Kaur S, Mann S (2009) Effect of salicylic and abscisic acid administered through detached tillers on antioxidant system in developing wheat grains under heat stress. Acta Physiol Plant 31:1091–1096. doi:10.1007/s11738-009-0335-y
Boscolo P, Menossi M, Jorge R (2003) Aluminum-induced oxidative stress in maize. Phytochemistry 62:181–189. doi:10.1016/S0031-9422(02)00491-0
Dhindsa R, Plumb-Dhindsa P, Thorpe T (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93
Dipierro N, Mondelli D, Paciolla C, Brunetti G, Dipierro S (2005) Changes in the ascorbate system in the response of pumpkin (Cucurbita pepo L.) roots to aluminium stress. J Plant Physiol 162:529–536. doi:10.1016/j.jplph.2004.06.008
Dong B, Sang W, Jiang X, Zhou J, Kong F, Hu W, Wang L (2002) Effects of aluminum on physiological metabolism and antioxidant system of wheat (Triticum aestivum L.). Chemosphere 47:87–92. doi:10.1016/S0045-6535(01)00210-7
Foyer C, Lopez-Delgado H, Dat J, Scott I (1997) Hydrogen peroxide-and glutathione-associated mechanisms of acclimatory stress tolerance and signalling. Physiol Plant 100:241–254. doi:10.1111/j.1399-3054.1997.tb04780.x
Fridovich I (1978) The biology of oxygen radicals. Sci 201:875–880
Ghanati F, Morita A, Yokota H (2005) Effects of aluminum on the growth of tea plant and activation of antioxidant system. Plant Soil 276:133–141. doi:10.1007/s11104-005-3697-y
Hoagland D, Arnon D (1950) The water culture method for growing plants without soil. Calif Agric Exp Stn Circ 347:1–32
Kochian L (1995) Cellular mechanisms of aluminum toxicity and resistance in plants. Annu Rev Plant Biol 46:237–260. doi:10.1146/annurev.pp.46.060195.001321
Liau Y, Wen L, Shaw J, Lin C (2007) A highly stable cambialistic-superoxide dismutase from Antrodia camphorata: expression in yeast and enzyme properties. J Biotechnol 131:84–91. doi:10.1016/j.jbiotec.2007.05.021
Ma J, Hiradate S, Nomoto K, Iwashita T, Matsumoto H (1997a) Internal detoxification mechanism of Al in hydrangea (identification of Al form in the leaves). Plant Physiol 113:1033
Ma J, Zheng S, Matsumoto H, Hiradate S (1997b) Detoxifying aluminium with buckwheat. Nature 390:569–570. doi:10.1038/37518
Mascher R, Lippmann B, Holzinger S, Bergmann H (2002) Arsenate toxicity: effects on oxidative stress response molecules and enzymes in red clover plants. Plant Sci (Limerick) 163:961–969. doi:10.1016/S0168-9452(02)00245-5
Matsumoto H, Hirasawa E, Morimura S, Takahashi E (1976) Localization of aluminum in tea leaves. Plant Cell Physiol 17:627–631
Mohan Murali Achary V, Jena S, Panda K, Panda B (2008) Aluminium induced oxidative stress and DNA damage in root cells of Allium cepa L. Ecotoxicol Environ Saf 70:300–310. doi:10.1016/j.ecoenv.2007.10.022
Morita A, Horie H, Fujii Y, Takatsu S, Watanabe N, Yagi A, Yokota H (2004) Chemical forms of aluminum in xylem sap of tea plants (Camellia sinensis L.). Phytochemistry 65:2775–2780. doi:10.1016/j.phytochem.2004.08.043
Morita A, Yanagisawa O, Takatsu S, Maeda S, Hiradate S (2008) Mechanism for the detoxification of aluminum in roots of tea plant (Camellia sinensis (L.) Kuntze). Phytochemistry 69:147–153. doi:10.1016/j.phytochem.2007.06.007
Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867
Patterson B, MacRae E, Ferguson I (1984) Estimation of hydrogen peroxide in plant extracts using titanium (IV). Anal Biochem 139:487–492
Pereira G, Molina S, Lea P, Azevedo R (2002) Activity of antioxidant enzymes in response to cadmium in Crotalaria juncea. Plant Soil 239:123–132. doi:10.1023/A:1014951524286
Ramiro D, Guerreiro-Filho O, Mazzafera P (2006) Phenol contents, oxidase activities, and the resistance of coffee to the leaf miner Leucoptera coffeella. J Chem Ecol 32:1977–1988. doi:10.1007/s10886-006-9122-z
Shinogi T, Suzuki T, Kurihara T, Narusaka Y, Park P (2003) Microscopic detection of reactive oxygen species generation in the compatible and incompatible interactions of Alternaria alternata Japanese pear pathotype and host plants. J Gen Plant Pathol 69:7–16. doi:10.1007/s10327-002-0013-z
Tahara K, Yamanoshita T, Norisada M, Hasegawa I, Kashima H, Sasaki S, Kojima K (2008) Aluminum distribution and reactive oxygen species accumulation in root tips of two Melaleuca trees differing in aluminum resistance. Plant Soil 307:167–178. doi:10.1007/s11104-008-9593-5
Tan S, Zhu M, Zhang Q (2010) Physiological responses of bermudagrass (Cynodon dactylon) to submergence. Acta Physiol Plant 32:133–140. doi:10.1007/s11738-009-0388-y
Tasgín E, Atící O, Nalbanto B (2003) Effects of salicylic acid and cold on freezing tolerance in winter wheat leaves. Plant Growth Regul 41:231–236. doi:10.1023/B:GROW.0000007504.41476.c2
Watanabe T, Osaki M, Yoshihara T, Tadano T (1998) Distribution and chemical speciation of aluminum in the Al accumulator plant, Melastoma malabathricum L. Plant Soil 201:165–173. doi:10.1023/A:1004341415878
Xu S, Li J, Zhang X, Wei H, Cui L (2006) Effects of heat acclimation pretreatment on changes of membrane lipid peroxidation, antioxidant metabolites, and ultrastructure of chloroplasts in two cool-season turfgrass species under heat stress. Environ Exp Bot 56:274–285. doi:10.1016/j.envexpbot.2005.03.002
Xu W, Shi W, Ueda A, Takabe T (2008) Mechanisms of salt tolerance in transgenic Arabidopsis thaliana carrying a peroxisomal ascorbate peroxidase gene from barley. Pedosphere 18:486–495. doi:10.1016/S1002-0160(08)60039-9
Zhu Z, Wei G, Li J, Qian Q, Yu J (2004) Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt-stressed cucumber (Cucumis sativus L.). Plant Sci 167:527–533. doi:10.1016/j.plantsci.2004.04.020
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Communicated by G. Bartosz.
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Li, C., Xu, H., Xu, J. et al. Effects of aluminium on ultrastructure and antioxidant activity in leaves of tea plant. Acta Physiol Plant 33, 973–978 (2011). https://doi.org/10.1007/s11738-010-0629-0
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DOI: https://doi.org/10.1007/s11738-010-0629-0