Summary
To study the relationship between the late postmortem interval (PMI) and trimethylamine-nitrogen (TMA-N) in postmortem tissues of cadaver, TMA-N in muscles, livers and kidneys of rats was measured at different postmortem intervals (PMI) by using a modified spectrophotometric method. The results indicated that the detection sensitivity of TMA-N was 1 mg/L, and there was a good linear correlation between the value of absorbance (A value) and TMA-N at the concentration of 1–10 mg/L (R 2 = 0.9991). Although TMA variation in muscles was different from that in inner organs during the time since death, TMA-N changes in cadaver tissues was positively correlated with PMI. During 2 to 7 d since death, the best correlation between PMI and TMA-N concentration was found in muscles. With PMI as an independent variable, the cubic polynomial regression equation was y= −0.457x3+6.519x2−24.574x+27.207 (R 2=0.969). During 3 to 8 days since death, PMI was best correlated with TMA-N concentration in inner organs. With PMI as the independent variable, the cubic polynomial regression equation was y=0.509x3−9.153x2+55.727x−95.819 (R 2=0.953). It was concluded that TMA-N in tissues could be used as a new estimator for late PMI. The method used in this study offered advantages such as accuracy, sensitivity, little samples required and wide PMI estimation.
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Qian LIU, female, born in 1980, Doctorial Candidate
This project was supported by a grant from the Health Department of Hubei Province (No. JX2B02).
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Liu, Q., Cai, X., Liu, Y. et al. Spectrophotometric determination of trimethylamine-nitrogen in cadaver tissues for the estimation of late postmortem interval: A pilot study. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 28, 630–633 (2008). https://doi.org/10.1007/s11596-008-0603-x
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DOI: https://doi.org/10.1007/s11596-008-0603-x