Tree Genetics & Genomes

, Volume 9, Issue 3, pp 829–840

Complex origin of Trinitario-type Theobroma cacao (Malvaceae) from Trinidad and Tobago revealed using plastid genomics

  • Ji Yong Yang
  • Moira Scascitelli
  • Lambert A. Motilal
  • Saemundur Sveinsson
  • Johannes M. M. Engels
  • Nolan C. Kane
  • Hannes Dempewolf
  • Dapeng Zhang
  • Kamaldeo Maharaj
  • Quentin C. B. Cronk
Original Paper

DOI: 10.1007/s11295-013-0601-4

Cite this article as:
Yang, J.Y., Scascitelli, M., Motilal, L.A. et al. Tree Genetics & Genomes (2013) 9: 829. doi:10.1007/s11295-013-0601-4

Abstract

Trinidad and Tobago has a long history of producing high-quality cacao (Theobroma cacao L.). Cacao genotypes in Trinidad and Tobago are of a highly distinctive kind, the so-called “Trinitario” cultivar group, widely considered to be of elite quality. The origin of Trinitario cacao is unclear, although it is generally considered to be of hybrid origin. We used massive parallel sequencing to identify polymorphic plastidic single nucleotide polymorphisms (cpSNPs) and polymorphic plastidic simple sequence repeats (cpSSRs) in order to determine the origin of the Trinitario cultivar group by comparing patterns of polymorphism to a reference set of ten completely sequenced chloroplast genomes (nine T. cacao and one outgroup, T. grandiflorum (Willd. ex Spreng.) Schum). Only three cpSNP haplotypes were present in the Trinitario cultivars sampled, each highly distinctive and corresponding to reference genotypes for the Criollo (CRI), Upper Amazon Forastero (UAF) and Lower Amazon Forastero (LAF) varietal groups. These three cpSNP haplotypes likely represent the founding lineages of cacao to Trinidad and Tobago. The cpSSRs were more variable with eight haplotypes, but these clustered into three groups corresponding to the three cpSNP haplotypes. The most common haplotype found in farms of Trinidad and Tobago was LAF, followed by UAF and then CRI. We conclude that the Trinitario cultivar group is of complex hybrid origin and has derived from at least three original introduction events.

Keywords

Theobroma cacao Chloroplast Microsatellites Single nucleotide polymorphisms Trinitario 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Ji Yong Yang
    • 1
  • Moira Scascitelli
    • 1
  • Lambert A. Motilal
    • 2
  • Saemundur Sveinsson
    • 1
  • Johannes M. M. Engels
    • 3
  • Nolan C. Kane
    • 1
  • Hannes Dempewolf
    • 1
  • Dapeng Zhang
    • 4
  • Kamaldeo Maharaj
    • 5
  • Quentin C. B. Cronk
    • 1
  1. 1.Department of BotanyUniversity of British ColumbiaVancouverCanada
  2. 2.Cocoa Research UnitThe University of the West Indies, St. Augustine, TrinidadWest IndiesRepublic of Trinidad and Tobago
  3. 3.Biodiversity InternationalRomeItaly
  4. 4.SPCL, USDA-ARSBeltsvilleUSA
  5. 5.Ministry of Agriculture, Food Production Land, and Marine Resources AffairsCentral Experiment Station, Centeno, Via Arima P.O.West IndiesRepublic of Trinidad and Tobago

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