Abstract
The Klebsiella sp. strain ATCC13883T capable of degrading carbofuran phenol (2,3-dihydro-2,2-dimethylbenzofuran-7-ol) has been separated from the soil by enrichment culture technique and immobilized in various, namely polyurethane foam (PUF), polyacrylamide, alginate, agar and alginate-bentonite clay-powdered activated charcoal (PAC). The degradation rates of 20 and 30 mM carbofuran phenol by free and immobilized cells in batch and semi-continuous shaken cultures were compared. The PUF-immobilized cells achieved higher degradation rates in a shorter time than freely suspended cells and the cells immobilized in polyacrylamide, alginate and agar. The PUF- and alginate-bentonite clay-PAC-immobilized cells could be reused for more than 36 cycles, polyacrylamide-entrapped cells for 20 cycles and alginate-bentonite-PAC 28 cycles, without losing any degradation capacity and showed better tolerance to pH, temperature and concentration changes than free cells. These results showed that cells immobilized in modified alginate-bentonite-PAC immobilizers tolerated and completely degraded carbofuran phenol at initial concentrations of 20 and 30 mM and also higher. Such a bacterial strain could be used for bioremediation of environments contaminated with phenolic compounds.
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References
Bettman H, Rehm HJ (1984) Degradation of phenol by polymer entrapped microorganisms. Appl Microbiol Biotechnol 20:285–290. doi:10.1007/BF00270587
Cassidy MB, Lee H, Trevors JT (1996) Environmental applications of immobilized microbial cells: a review. J Ind Microbiol 16:79–101. doi:10.1007/BF01570068
Chapalmandugu S, Chaudhry GR (1992) Microbial and biotechnological aspects of metabolism of carbamates and organophosphates. Crit Rev Biotechnol 12:357–389
Chaudhry GR, Ali AN (1988) Bacterial metabolism of carbofuran. Appl Environ Microbiol 54:1414–1419
Chaudhry GR, Mateen A, Kaskar B, Bloda M, Riazuddin S (2002a) Purification, biochemical characterization of the carbamate hydrolase from Pseudomonas sp. 50432. Biotechnol Appl Biochem 36:63–70
Chaudhry GR, Mateen A, Kaskar B, Sardessai M, Bloda MA, Bhatti R, Walia SK (2002b) Induction of carbofuran oxidation to 4-hydroxycarbofuran by Pseudomonas sp. 50432. FEMS Microbiol Lett 214:171–176
Chung TP, Tseng HY, Juang RS (2003) Mass transfer effect and intermediate detection for phenol degradation in immobilized Pseudomonas putida systems. Process Biochem 38:1497–1507
D’Souza SF (2002) Trends in immobilized enzyme and cell technology. Indian J Biotechnol 1:321–328
Desaint S, Hartmann A, Parekh NR, Fournier JC (2000) Genetic diversity of carbofuran-degrading soil bacteria. FEMS Microbiol Ecol 34:173–180
Feng X, Ou LT, Ogram AV (1997) Plasmid-mediated mineralization of carbofuran by Sphingomonas sp. CF06. Appl Environ Microbiol 63:1332–1337
Hall DO, Rao KK (1989) Immobilized photosynthetic membranes and cells for the production of fuels and chemicals. Chim Oggi 1:41–47
Jiyoung S, Junho J, Sang-Don K, Suil K, Jaehong H, Hor-Gil H (2007) Fungal biodegradation of carbofuran and carbofuran phenol by the fungus Mucor ramannianus: identification of metabolites. Water Sci Technol 55:163–167
Jonathan W (1988) Methods of immobilization of microbial cells. J Microb Methods 8:91–102
Kamanavalli CM, Ninnekar HZ (2000) Biodegradation of Propoxur by Pseudomonas species. World J Microbiol Biotechnol 16:329–331
Marwaha SS, Kennedy JF, Khanna PK, Tewari HK, Redhu A (1989) In physiology of Immobilized cells. Proceedings of an international symposium. Elsevier Science Publishers, Wageningen, p 265
Nadal M, Sobhi B, Isam S (2007) Effect of adsorption and bead size of immobilized biomass on the rate of biodegradation of phenol at high concentration levels. Ind Eng Chem Res 46:6820–6824
Ramanand K, Sharmila M, Singh N, Sethunathan N (1991) Metabolism of carbamate insecticides by resting cells and cell-free preparations of a soil bacterium, Arthrobacter sp. Bull Environ Contam Toxicol 46:380–386
Seubert W (1960) Determination of isoprenoid compounds by microorganisms. I. Isolation and characterization of an isoprenoid-degrading bacterium, Pseudomonas citronellolis, new species. J Bacteriol 79:426–434
Tomasek PH, Karns J (1989) Cloning of a carbofuran hydrolase gene from Achromonobacter sp. strain WM111 and its expression in gram-negative bacteria. J Bacteriol 171:4038–4044
Trevors JT, Van Elsas JD, Lee H, Van Overbeek LS (1992) Use of alginate and other carriers for encapsulation of microbial cells for use in soil. Microb Release 1:61–69
Trevors JT, Lee H, Wolters AC, Van Elsas JD (1993) Survival of alginate encapsulated Pseudomonas fluorescens cells in soil. Appl Environ Microbiol 30:637
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The authors wish to express all their gratitude to University Sophisticated Instrumentation Centre (USIC), Karnatak University, Dharwad for providing instrumental facilities.
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Kadakol, J.C., Kamanavalli, C.M. & Shouche, Y. Biodegradation of Carbofuran phenol by free and immobilized cells of Klebsiella pneumoniae ATCC13883T. World J Microbiol Biotechnol 27, 25–29 (2011). https://doi.org/10.1007/s11274-010-0422-7
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DOI: https://doi.org/10.1007/s11274-010-0422-7