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Distribution of some virulence related-properties of Vibrio alginolyticus strains isolated from Mediterranean seawater (Bay of Khenis, Tunisia): investigation of eight Vibrio cholerae virulence genes

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Abstract

This study characterizes 28 Vibrio alginolyticus strains isolated from seawater from the Seacoast of Monastir (Khenis; Tunisia). V. alginolyticus were isolated using the TCBS modified agar plates and the biochemical activities were tested using RapID NF plus Strips. Proteases activities, hemolysis, antibiotics susceptibility, and adhesion to fish mucus and epithelial cell lines (Hep-2 and Caco-2) were also investigated. Eight Vibrio cholerae virulence genes (toxR, toxS, toxRS, toxT, ctxA, vpi, ace, zot) were investigated by PCR in genomes of V. alginolyticus strains. Most of the studied strains were β-haemolytic and produce many proteolytic enzymes. All isolates described here were resistant to several antibiotics tested. Six strains were able to adhere strongly to both Hep-2 and Caco-2 cell lines. The PCR investigation of V. cholerae genes showed a large distribution among the genomes of all V. alginolyticus strains. The toxR operon was found in 9 V. alginolyticus strains out of 28 studied. Only one strain was positive for the toxS and toxRS respectively. Five strains showed a positive amplification for the virulence pathogenic island (vpi), seven for the toxT, 3 for the ctxA and 9 for the Zonula occludens toxin (zot). The bay of Khenis harbors different genotypes of V. alginolyticus strains who inheritated several virulence genes from autochthones bacteria such as V. cholerae. These strains were able to produce several virulence enzymes and exhibit a high power to adhere to human epithelial cells and fish mucus.

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References

  • Baffone W, Vittoria E, Campana R et al (2005) Occurrence and expression of virulence related properties by environmental Vibrio spp. In in vitro and in vivo systems. Food Control 16:451–457

    Article  CAS  Google Scholar 

  • Baffone W, Renato T, Luigi P et al (2006) Detection of free-living and plankton-bound vibrios in coastal waters of the Adriatic Sea (Italy) and study of their pathogenicity-associated properties. Environ Microbiol 8(7):1299–1305

    Article  CAS  Google Scholar 

  • Bakhrouf A, Ben Ouada H, Oueslati R (1992) Essai d’identification de deux vibrions isolés dans une zone de pisciculture. Microbiologie Hygiène Alimentaire 4(9):3–6

    Google Scholar 

  • Bakhrouf A, Jeddi M, Ben Ouada H (1995) Essai de traitement des vibrioses du loup Dicentrarchus labrax dans une zone de pisciculture, à Monastir, Tunisie. Marine Life 5(2):47–54

    Google Scholar 

  • Balebona MC, Morinigo MA, Borrego JJ (1995) Role of extracellular products in the pathogenicity of Vibrio strains on cultured gilt-head sea bream (Sparus aurata). Microbiologia 11(4):439–446

    CAS  Google Scholar 

  • Ben Kahla-Nakbi A, Chaieb K, Besbes A et al (2006) Virulence and enteriobacterial repetitive intergenic consensus PCR of Vibrio alginolyticus strains isolated from Tunisian cultured gilthead sea bream and sea bass outbreaks. Vet Microbiol 117(2–4):321–327

    Article  CAS  Google Scholar 

  • Blake PA, Merson MH, Weaver RE et al (1979) Disease caused by a marine Vibrio. Clinical characteristics and epidemiology. N Engl J Med 300:1–5

    Article  CAS  Google Scholar 

  • Bordas MA, Balebona MC, Zorilla I et al (1996) Kinetics of adhesion of selected fish-pathogenic Vibrio strains to skin mucus of gilt-head sea bream. Appl Environ Microbiol 62:3650–3654

    CAS  Google Scholar 

  • Boyd EF, Moyer KE, Shi L et al (2000) Infectious CTXphi and the Vibrio pathogenicity island prophage in Vibrio mimicus: evidence for recent horizontal transfer between V. mimicus and V. cholerae. Infect Immun 68:1507–1513

    Article  CAS  Google Scholar 

  • Bravo Farinas L, Monteboada RJ, Gomez Blanco M et al (1992) Identification of microorganism of the genus Vibrio. Rev Cubana Med Trop 43(2):107–110

    Google Scholar 

  • Cavallo RA, Stabili L (2004) Culturable vibrios biodiversity in the Northan Ionian Sea (Italy). SCI MAR 68(1):23–29

    Article  Google Scholar 

  • Cavallo JD, Chardon H, Chidiac C et al (2006) Comité de l’antibiogramme de la société Française de Microbiologie (Communiqué 2006)

  • Chakraborty S, Nair GB, Shinoda S (1997) Pathogenic Vibrios in the natural aquatic environment. Rev Environ Health 12(2):63–80

    CAS  Google Scholar 

  • Chowdhury MAR, Yamanaka H, Miyoshi S et al (1990) Ecology and seasonal distribution of Vibrio parahaemolyticus in aquatic environments of a temperate region. FEMS Microbiol Ecol 74:1–110

    Article  Google Scholar 

  • Colombo MM, Mastrandea S, Santona A (1994) Distribution of the ace, zot and ctxA genes in clinical and environmental Vibrio cholerae. J Infect Dis 170:750–751

    CAS  Google Scholar 

  • Deriu A, Mura A, Delogu D et al (2005) Isolamento ed identificazione di diverse specie di Vibrio spp. Da acque e molluschi prelevati nel golfo di Olbia. L’igiene Moderna 123:17–25

    Google Scholar 

  • Deriu A, Sechi LA, Molicotti P et al (2002) Virulence genes in halophilic Vibrio spp. Isolated in common mussels. Microbiologica 25:93–96

    CAS  Google Scholar 

  • English VL, Lindberg RB (1977) Isolation of Vibrio alginolyticus from wounds and blood of a burn patient. Am J Med Technol 43:989–993

    CAS  Google Scholar 

  • Farina C, Gnecchi F, Luzzi I et al (2000) Vibrio cholerae O2 as a causative of skin lesion in a tourist returning from Tunisia. J Travel Med 7:92–94

    Article  CAS  Google Scholar 

  • Faruque SM, Manujendra A, Saha N (1998) Analysis of clinical and environmental strains of non-toxigenic Vibrio cholerae for susceptibility to CTXØ: Molecular basis for origination of new strains with epidemic potential. Infect Immun 66:5819–5825

    CAS  Google Scholar 

  • Field PI, Popovic P, Wachsmuth K et al (1992) Use of Polymerase Chain Reaction for detection of toxigenic Vibrio cholerae O1 strains from the Latin American cholera epidemic. J Clin Microbiol 30:2118–2121

    Google Scholar 

  • Fluharty DM, Packard WL (1967) Differentiation of Gram-positive and Gram-negative bacteria without staining. Am J Vet Clin Pathol 1:31–35

    Google Scholar 

  • Gomez JM, Fajardo R, Patiño JF et al (2003) Necrotizing Fasciitis due to Vibrio alginolyticus in an Immunocompetent Patient. J Clin Microbiol 41(7):3427–3429

    Article  Google Scholar 

  • Hare P, Scott-Burden T, Wodds DR (1983) Characterization of extracellular aklaline proteases and collagenase induction in Vibrio alginolyticus. J Gen Microbiol 129:1141–1143

    CAS  Google Scholar 

  • Kelly MT (1982) Effect of temperature and salinity on Vibrio (Beneckea) vulnificus occurrence in a Gulf Coast environment. Appl Environ Microbiol 44:820–824

    CAS  Google Scholar 

  • Lee KK (1995) Pathogenesis studies on Vibrio alginolyticus in the grouper, Epinephelus malabaricus Bloch et Schneider. Microb Pathog 19:39–48

    CAS  Google Scholar 

  • Li J, Yie J, Foo RWT et al (1998) Antibiotic resistance and plasmid profiles of Vibrio isolates from cultured silver sea bream, Sparus sarba. Mar Pollut Bull 39:245–249

    Article  Google Scholar 

  • Lyer L, Vadivelu J, Puthucheary SD (2000) Detection of virulence associated genes, haemolysin and protease amongst Vibrio cholerae isolated in Malysia. Epidemiol Infect 125:27–34

    Article  Google Scholar 

  • Manning PA, Clark CA, Focarcta T (1999) Gene capture in Vibrio cholerae. Trends Microbiol 7:93–95

    Article  CAS  Google Scholar 

  • Masini L, De Grandis G, Principi F et al (2007) Research and characterization of pathogenic vibrios from bathing water along the Conero Riviera (Central Italy). Water Res 41:4031–4040

    Article  CAS  Google Scholar 

  • Montanari MP, Pruzzo C, Pane L et al (1999) Vibriosis associated with plankton in a coastal zone of the Adriatic Sea (Italy). FEMS Microbiol Ecol 29(3):241–247

    Article  CAS  Google Scholar 

  • NCCLS - National Committee for Clinical Laboratory Standards (2002) Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. Tentative standards. M31-T. Document M31-T. Wayne

  • Ochman H, Lawrence JG, Groisman EA (2000) Lateral gene transfer and the nature of bacterial innovation. Nature 405:299–304

    Article  CAS  Google Scholar 

  • Olafsen JA (2001) Interaction between fish larvae and bacteria in Marine Aquaculture. Aquaculture 200:223–247

    Article  Google Scholar 

  • Opal SM, Saxon JR (1986) Intracranial infection by Vibrio alginolyticus following injury in salt water. J Clin Microbiol 23(2):373–374

    CAS  Google Scholar 

  • Ottaviani D, Bacchiocchi I, Masini L et al (2001) Antimicrobial susceptibility of potentially pathogenic halophilic vibrios isolated from seafood. Int J Antimicrob Agents 18:135–140

    Article  CAS  Google Scholar 

  • Piersimoni C, Morbiducci V, Scalise C (1991) Vibrio cholerae gastroenteritis and bacteremia. Lancet 337:791–792

    Article  CAS  Google Scholar 

  • Rhodes G, Huys G, Swings J et al (2000) Distribution of oxytetracycline resistance plasmids between aeromonads in hospital and aquaculture environments: implication of Tn 1721 in dissemination of the tetracycline resistance determinant TetA. Appl Environ Microbiol 66:3883–3890

    Article  CAS  Google Scholar 

  • Ripabelli G, Sammarco ML, McLauchlin J et al (2003) Molecular characterisation and antimicrobial resistance of Vibrio vulnificus and Vibrio alginolyticus isolated from mussels (Mytilus galloprovincialis). Syst Appl Microbiol 26(1):119–126

    Article  CAS  Google Scholar 

  • Sally JR, Richard CT (1975) Isolation of Vibrio alginolyticus from Wound Infections. J Clin Microbiol 2(6):556–558

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY

    Google Scholar 

  • Sechi LA, Duprè A, Deriu A et al (2000) Distribution of Vibrio cholerae genes among different Vibrio species isolated in Sardinia, Italy. J Appl Microbiol 88:475–481

    Article  CAS  Google Scholar 

  • Snoussi M, Chaieb K, Rouabhia M et al (2006) Quantitative study, identification and antibiotics sensitivity of some Vibrionaceae associated to a marine fish hatchery. Ann Microbiol 56(4):289–293

    CAS  Google Scholar 

  • Snoussi M, Hajlaoui H, Noumi E et al (2008) Phenotypic and genetic diversity of Vibrio alginolyticus strains recovered from juveniles and older Sparus aurata reared in a Tunisian marine farm. Ann Microbiol 58(1):141–146

    Article  CAS  Google Scholar 

  • Thompson FL, Iida T, Swings J (2004) Biodiversity of vibrios. Microbiol Mol Biol Rev 68:3403–3431

    Article  CAS  Google Scholar 

  • Tülay A, Gülşen T (2002) Vibriosis in Gilthead Sea Bream (Sparus aurata L.) in farms in the Aegean Sea Coast of Turkey. Turkish J Fish Aquat Sci 2:89–91

    Google Scholar 

  • Vanderberghe J, Thompson FL, Gomez-Gil B et al (2003) Phenotypic diversity amongst Vibrio isolates from marine aquaculture systems. Aquaculture 219(1):9–20

    Article  Google Scholar 

  • Waldor MK, Mekalanos JJ (1996) Lysogenic conversion by a filamentous phage encoding cholera toxin. Science 272:1910–1914

    Article  CAS  Google Scholar 

  • Wong SM, Carroll PA, Rahme LG et al (1998) Modulation of expression of the ToxR regulon in Vibrio cholerae by a member of the two component family of response regulators. Infect Immun 66:5854–5861

    CAS  Google Scholar 

  • Xie ZY, Hu CQ, Chen C et al (2005) Investigation of seven Vibrio virulence genes among Vibrio alginolyticus and Vibrio parahaemolyticus strains from the coastal mariculture systems in Guangdong, China. Lett Appl Microbiol 41:202–207

    Article  CAS  Google Scholar 

  • Zanetti S, Deriu A, Duprè I et al (1999) Differentiation of Vibrio alginolyticus strains isolated from Sardinian waters by ribotyping and a new rapid PCR fingerprinting method. Appl Environ Microbiol 65:1871–1875

    CAS  Google Scholar 

  • Zanetti S, Deriu A, Volterra L et al (2000) Virulence factors in Vibrio alginolyticus strains isolated from aquatic environments. Ann Ig 12(6):487–491

    CAS  Google Scholar 

  • Zanetti S, Spanu T, Deriu A et al (2001) In vitro susceptibility of Vibrio spp. Isolated from the environment. Int J Antimicrob Agents 17(6):407–409

    Article  CAS  Google Scholar 

  • Zorrilla I, Moriñigo MA, Castro D et al (2003) Intraspecific characterization of Vibrio alginolyticus isolates recovered from cultured fish in Spain. J Appl Microbiol 95:1106–1116

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des Produits, Department of Microbiology, Faculty of Pharmacy, Rue Avicenne, Monastir, 5000, Tunisia

    Mejdi Snoussi, Emira Noumi & Amina Bakhrouf

  2. Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Universita di Sassari, Viale San Pietro 43/b, 07100, Sassari, Italy

    Mejdi Snoussi, Donatella Usai, Leonardo Antonio Sechi & Stefania Zanetti

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  1. Mejdi Snoussi
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  2. Emira Noumi
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  4. Leonardo Antonio Sechi
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  5. Stefania Zanetti
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  6. Amina Bakhrouf
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Correspondence to Mejdi Snoussi.

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Snoussi, M., Noumi, E., Usai, D. et al. Distribution of some virulence related-properties of Vibrio alginolyticus strains isolated from Mediterranean seawater (Bay of Khenis, Tunisia): investigation of eight Vibrio cholerae virulence genes. World J Microbiol Biotechnol 24, 2133–2141 (2008). https://doi.org/10.1007/s11274-008-9719-1

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  • DOI: https://doi.org/10.1007/s11274-008-9719-1

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