Skip to main content
SpringerLink
Log in

Slaughterhouse prevalence of ovine paratuberculosis in Southern India

  • Short Communications
  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

Ovine paratuberculosis is a threat to small animal holders in terms of economic loss such as reduced growth performance and early culling. In order to study the slaughterhouse prevalence of ovine paratuberculosis, the slaughterhouse sheep samples (which are poor in body condition) collected over a period of two and half years from 1,034 suspected male sheep (poor in body condition) and 40 normal sheep (good body condition and subsequently negative by all the diagnostic tests employed) aged between 16 and 18 months were slaughtered at various abattoirs of Tamil Nadu. All the sheep taken in this study were maintained in almost same management conditions. DNA was extracted from 1,034 intestinal tissue and mesenteric lymph node and 121 were positive by IS 900 PCR. One hundred ten and 56 were positive by absorbed ELISA and Ziehl–Neelsen staining, respectively. In histopathology, 28 animals showed gross lesions of paratuberculosis infection (20—multibacillary and 8—paucibacillary forms). Out of 1,034 sheep tissues cultured, 32 showed cultural growth in Middlebrook 7H9 and 26 in Herrold's egg yolk medium. None of the 40 normal sheep were positive by any of the tests employed. In general, the mean body weight of paratuberculosis-affected animal either by any one of the tests employed was less than the non-affected sheep. The approximate economic loss per sheep/farmer/year is around Rs 1,840 (US$ 38.33) in paratuberculosis-affected sheep.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Bush, R.D., Windsor, P.A., Toribio J-Alml., Webster, S.R., 2005. Modelling the on-farm financial impact of ovine Johne's disease in Australia. 8th International colloquium on paratuberculosis, Copenhagen, Denmark, August 14–17, pp 17.

  • Collins, D.M., Hilbink, F., West, D.M., Hosie, B.D., Cooke, M.M., de Lisle, G.W., 1993. Investigation of Mycobacterium paratuberculosis in sheep by faecal culture, DNA characterisation and the polymerase chain reaction. Veterinary Record, 133, 599–600.

    PubMed  CAS  Google Scholar 

  • Collins, D. M., De, Z. M., Cavaignac, S. M.,2002. Mycobacterium avium subsp. paratuberculosis strains from cattle and sheep can be distinguished by a PCR test based on a novel DNA sequence difference. Journal of Clinical Microbiology, 40, 4760–4762.

    Article  PubMed  CAS  Google Scholar 

  • Dimareli-Malli, Z., Stevenson, K., Sarris, K ., Sossidou, K., 2009. Study of microbiological and molecular typing aspects of paratuberculosis in sheep and goats in Northern Greece, Transboundary and Emerging Diseases, 56, 285–290.

    Article  PubMed  CAS  Google Scholar 

  • FAOSTAT. 2010. FAO year book.

  • Juste, R.A., Saez de Ocariz, C., Marco, J.C., Badiola, J.J., 1988. Diagnosis of clinical paratuberculosis in sheep: a comparison of pathological, bacteriological and serological methods. In: Thorel, M.F., Merkal, R.S. (Eds.), Proc. II Int. Coll. PTBC. Maisons-Alfort, pp. 220–226.

  • Koenig, G.J., Hoffsis, G.F., Shulaw, W.P., Beth-Nielsen, S., Rings, D.M., St-Jean, G., 1993. Isolation of Mycobacterium paratuberculosis from mononuclear cells in tissues, blood and mammary glands of cows with advanced paratuberculosis. American Journal Veterinary. Research, 54, 1441–1445.

    CAS  Google Scholar 

  • Maresca, C., Giorgi, G., Feliziani, F., et al., 1999. Indagine epidemiologica sulla paratubercolosi ovina nel territorio di una A.S.L. della regione Umbria, Large Animals Review, 5, 71–74.

    Google Scholar 

  • Mercier, P., Baudry, C., Beaudeau, F., 2010. Estimated prevalence of Mycobacterium aviumsubspecies paratuberculosis infection in herds of dairy goats in France. Veterinary Record, 167, 412–415.

    Article  PubMed  CAS  Google Scholar 

  • Nebbia, P., Robino, P., Zoppi, S., Meneghi, D.D., 2006. Detection and excretion pattern of Mycobacterium avium subspecies paratuberculosis in milk of asymptomatic sheep and goats by Nested-PCR. Small Ruminant Research, 66, 116–120.

    Article  Google Scholar 

  • Ott, S.L., Wells, S.J., Wagner, B.A.,1999. Herd-level economic losses associated with Johne’s disease on US dairy operations, Preventive Veterinary Medicine, 40, 179–192.

    Article  PubMed  CAS  Google Scholar 

  • Perez, V., Garcıa Marın, J. F., Badiola, J. J., 1996. Description and classification of different types of lesion associated with natural paratuberculosis infection in sheep. Journal of Comparative Pathology, 114, 107–122.

    Article  PubMed  CAS  Google Scholar 

  • Rita, A..A., Victor, N. N., Silvia, P., Luciana, P., Anastasia, D., Vincenzo, C., 2011. Ovine Paratuberculosis: A Seroprevalence Study in Dairy Flocks Reared in the Marche Region, Italy. Veterinary Medicine International. doi:10.4061/2011/782875

  • Sergeant, E. S. G., Baldock, F. C., 2002. The estimated prevalence of Johne’s disease infected sheep flocks in Australia, Australian Veterinary Journal, 80, 762–768.

    Article  PubMed  CAS  Google Scholar 

  • Sevilla, I., Singh, S.V., Garrido, J.M., Aduriz, G., Rodrıguez, S., Geijo, M.V., Whittington, R.J., Saunders, V.,Whitlock, R.H., Juste, R.A.., 2005. PCR-REA genotype of paratuberculosis strains isolated from different host species and geographical locations. Rev. Sci, OIE 24, 1061–1066.

    CAS  Google Scholar 

  • Singh, S.V., Singh, A.V., Singh, P.K., Gupta, V.K., Kumar, S., Vohra, J., 2007. Sero-prevalence of paratuberculosis in young kids using ‘Bison type’, Mycobacterium avium subsp. paratuberculosis antigen in plate ELISA. Small Ruminant Research, 70, 89–92.

    Article  Google Scholar 

  • Singh, P. K., Singh, S. V., Singh, A. V., Sohal, J. S., 2008. Screening of tissues and serum by culture, PCR and ELISA for the detection of Mycobacterium avium subspecies paratuberculosis from cases of clinical ovine Johne’s disease in farmer’s flocks. Indian Journal of Animal Sciences, 78, 1052–1056.

    CAS  Google Scholar 

  • Timms, V. J., Gehringer, M. M., Mitchell, H. M., Daskalopoulos, G., Neilan, B. A., 2011. Review: How accurately can we detect Mycobacterium avium subsp. paratuberculosis infection? Journal of Microbiological Methods, 85, 1–8.

    Article  PubMed  Google Scholar 

  • Vary, P.H., Andersen, P.R., Green, E., Taylor, J.H., McFalden, J.J., 1990. Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne’s disease. Journal of Clinical Microbiology. 28, 933–937.

    Google Scholar 

  • Villari, S., Castiglione, F., Monteverde, V., et al.,2009. Seroprevalence of Mycobacterium avium subsp. paratuberculosis (MAP) in ovine and caprine farms in Trapani, Sicily, in Proceedings of the 17th International Congress of Mediterranean Federation of Health and Production of Ruminants, Perugia, Italy, May 2009, pp. 117–118.

  • VinodhKumar, O.R., Gunaseelan, L., Sekar, M., Ronald, B.S.M., 2012a. Slaughter house prevalence of ovine paratuberculosis in Chennai city, India. 11th International colloquium on paratuberculosis. Sydney, Australia, Feb 5–10, 2012. pp 222.

  • VinodhKumar, O.R., Gunaseelan, L., Sekar, M., Ronald, B.S.M., 2012a. Prevalence of cattle paratuberculosis in farmer's field of Tamilnadu, India. 11th International colloquium on paratuberculosis. Sydney, Australia, Feb 5–10, 2012. pp 222.

  • VinodhKumar, O.R. and Gunaseelan, L., 2012b. Seroprevalence of ovine paratuberculosis. Indian Veterinary Journal, 89,124–126.

    Google Scholar 

  • Weber, M.F., Verhoeff, J., van Schaik, G., van Maanen, C., 2009. Evaluation of Ziehl–Neelsen stained faecal smear and ELISA as tools for surveillance of clinical paratuberculosis in cattle in the Netherlands. Preventive Veterinary Medicine, 92, 256–266.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Veterinary Epidemiology and Preventive Medicine, Madras Veterinary College, Chennai-7, India

    Obli Rajendran VinodhKumar, Lakshmanaswamy Gunaseelan, Berty Samuel Masilamony Ronald & Singamani Masilamoni Sakthivelan

  2. Animal Health, Central Sheep and Wool Research Institute, Avikanagar, Rajasthan, India

    Obli Rajendran VinodhKumar

  3. Veterinary Public Health, Madras Veterinary College, Chennai-7, India

    Lakshmanaswamy Gunaseelan

  4. Bacterial Vaccines, Madhavaram Milk Colony, Tanuvas, Chennai, India

    Berty Samuel Masilamony Ronald

  5. Veterinary Pathology, Madras Veterinary College, Chennai-7, India

    Singamani Masilamoni Sakthivelan

Authors
  1. Obli Rajendran VinodhKumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lakshmanaswamy Gunaseelan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Berty Samuel Masilamony Ronald
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Singamani Masilamoni Sakthivelan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Obli Rajendran VinodhKumar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

VinodhKumar, O.R., Gunaseelan, L., Ronald, B.S.M. et al. Slaughterhouse prevalence of ovine paratuberculosis in Southern India. Trop Anim Health Prod 45, 1063–1069 (2013). https://doi.org/10.1007/s11250-012-0321-z

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11250-012-0321-z

Keywords

Search

Navigation