Skip to main content
SpringerLink
Log in

Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs

  • Technical Update
  • Published:
Transgenic Research Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Reference

  • Ohtsuka M, Ogiwara S, Miura H, Mizutani A, Warita T, Sato M, Imai K, Hozumi K, Sato T, Tanaka M, Kimura M, Inoko H (2010) Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression. Nucleic Acids Res 38:e198

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

Funded by the Grant-in-Aid for Young Scientists (B) (20700368) to M.O. from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.

Author information

Authors and Affiliations

  1. Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan

    Masato Ohtsuka, Hiromi Miura, Minoru Kimura & Hidetoshi Inoko

  2. Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Yata 1111, Mishima, Shizuoka, 411-8540, Japan

    Hirofumi Nakaoka

  3. Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, 1-21-20 Korimoto, Kagoshima, Kagoshima, 890-0065, Japan

    Masahiro Sato

Authors
  1. Masato Ohtsuka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hiromi Miura
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hirofumi Nakaoka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Minoru Kimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Masahiro Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hidetoshi Inoko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masato Ohtsuka.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 150 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ohtsuka, M., Miura, H., Nakaoka, H. et al. Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs. Transgenic Res 21, 225–226 (2012). https://doi.org/10.1007/s11248-011-9505-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11248-011-9505-y

Keywords

Search

Navigation