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Comparable Attenuation of Aβ25–35-Induced Neurotoxicity by Quercitrin and 17β-Estradiol in Cultured Rat Hippocampal Neurons

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Abstract

In the present work, potential protective effects of quercitrin (a phytoestrogen) on Aβ-induced neurotoxicity in cultured rat hippocampal neurons were investigated in comparison with 17β-estradiol. Cell viability, oxidative status, and antioxidative potentials were used as comparative parameters. Co-exposure of cultured neurons to Aβ25–35 with either quercitrin or 17β-estradiol (50–100 μM) for 72 h attenuated Aβ25–35-induced neurotoxicity and lipid peroxidation, but not Aβ25–35-induced ROS accumulation. However, only 17β-estradiol counteracted a reduction in glutathione content and only quercitrin counteracted a reduction in glutathione peroxidase activity. Both compounds displayed no effects on superoxide dismutase activity. A specific estrogen receptor antagonist, ICI 182780, did not abolish neuroprotective effects of quercitrin and 17β-estradiol. These findings suggested that quercitrin and 17β-estradiol attenuated Aβ25–35-induced neurotoxicity in a comparable manner. Underlying neuroprotective mechanisms of both compounds were probably not related to estrogen receptor-mediated genomic mechanisms but might involve with their antioxidant and free radical scavenging properties.

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Acknowledgments

This study was supported partly by the Graduate Research Funds from the Graduate School, Chulalongkorn University.

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  1. Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand

    Sadudee Rattanajarasroj & Surachai Unchern

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  1. Sadudee Rattanajarasroj
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  2. Surachai Unchern
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Correspondence to Surachai Unchern.

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Rattanajarasroj, S., Unchern, S. Comparable Attenuation of Aβ25–35-Induced Neurotoxicity by Quercitrin and 17β-Estradiol in Cultured Rat Hippocampal Neurons. Neurochem Res 35, 1196–1205 (2010). https://doi.org/10.1007/s11064-010-0175-6

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