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Characterization of a diffuse intrinsic pontine glioma cell line: implications for future investigations and treatment

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Abstract

Diffuse intrinsic pontine gliomas arise almost exclusively in children, and despite advances in treatment, the majority of patients die within 2 years after initial diagnosis. Because of their infiltrative nature and anatomic location in an eloquent area of the brain, most pontine gliomas are treated without a surgical biopsy. The corresponding lack of tissue samples has resulted in a limited understanding of the underlying genetic and molecular biologic abnormalities associated with pontine gliomas, and is a substantial obstacle for the preclinical testing of targeted therapeutic agents for these tumors. We have established a human glioma cell line that originated from surgical biopsy performed on a patient with a pontine glioma. To insure sustainable in vitro propagation, tumor cells were modified with hTERT (human telomerase ribonucleoprotein reverse transcriptase), and with a luciferase reporter to enable non-invasive bioluminescence imaging. The hTERT modified cells are tumorigenic in athymic rodents, and produce brainstem tumors that recapitulate the infiltrative growth of brainstem gliomas in patients.

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Acknowledgments

This study was supported by the Pediatric Brain Tumor Foundation Institute Award to the University of California San Francisco, and Timmy’s Rainbow Foundation for Brainstem Tumor Research. The authors also thank Dr. Susan Baker (St. Jude’s Children’s Hospital and Medical Center) for allowing permission to examine copy number data from a published data set of pediatric astrocytic tumors.

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Authors and Affiliations

  1. Department of Neurological Surgery, Brain Tumor Research Center, University of California San Francisco, San Francisco, CA, 94143-0520, USA

    Rintaro Hashizume, Ivan Smirnov, Sharon Liu, Joanna J. Phillips, Jeanette Hyer, Tracy R. McKnight, Michael Prados, Anu Banerjee, Theodore Nicolaides, Sabine Mueller & Charles D. James

  2. Department of Pathology, University of California San Francisco, San Francisco, CA, 94143, USA

    Joanna J. Phillips

  3. Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA, 94143-0946, USA

    Tracy R. McKnight & Michael Wendland

  4. Department of Pediatrics, University of California San Francisco, San Francisco, CA, 94143, USA

    Anu Banerjee, Theodore Nicolaides, Sabine Mueller & Nalin Gupta

  5. Department of Neurology, University of California San Francisco, San Francisco, CA, 94143, USA

    Sabine Mueller

  6. Department of Neurological Surgery, Brain Tumor Research Center, University of California San Francisco, 505 Parnassus Avenue, Room M779, San Francisco, CA, 94143-0112, USA

    Nalin Gupta

Authors
  1. Rintaro Hashizume
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  2. Ivan Smirnov
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  3. Sharon Liu
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  4. Joanna J. Phillips
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  5. Jeanette Hyer
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  6. Tracy R. McKnight
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  7. Michael Wendland
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  8. Michael Prados
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  9. Anu Banerjee
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  10. Theodore Nicolaides
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  11. Sabine Mueller
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  12. Charles D. James
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  13. Nalin Gupta
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Corresponding author

Correspondence to Nalin Gupta.

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Hashizume, R., Smirnov, I., Liu, S. et al. Characterization of a diffuse intrinsic pontine glioma cell line: implications for future investigations and treatment. J Neurooncol 110, 305–313 (2012). https://doi.org/10.1007/s11060-012-0973-6

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  • DOI: https://doi.org/10.1007/s11060-012-0973-6

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