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Gene expression abnormalities in histologically normal breast epithelium from patients with luminal type of breast cancer

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Abstract

The gene expression profile of breast cancer has been described as a great breakthrough on the way to comprehend differences in cancer origin, behavior and therapy. However, gene expression profile in histologically normal epithelium (HNEpi) which could harbor genetic abnormalities predisposing breast tissue to develop malignancy was minor scope for scientists in the past. Thus, we aimed to analyze gene expressions in HNEpi and breast cancer tissue (BCTis) in order to establish its value as potential diagnostic marker for cancer development. We evaluated a panel of disease-specific genes in luminal type (A/B) of breast cancer and tumor surrounding HNEpi by qRT-PCR Array in 32 microdissected samples. There was 20.2 and 2.4 % deregulation rate in genes with at least 2-fold or 5-fold over-expression between luminal (A/B) type breast carcinomas and tumor surrounding HNEpi, respectively. The high-grade luminal carcinomas showed higher number of deregulated genes compared to low-grade cases (50.6 vs. 23.8 % with at least 2-fold deregulation rate). The main overexpressed genes in HNEpi were KLK5, SCGB1D2, GSN, EGFR and NGFR. The significant differences in gene expression between BCTis and HNEpi samples were revealed for BAG1, C3, CCNA2, CD44, FGF1, FOSL1, ID2, IL6R, NGFB, NGFR, PAPPA, PLAU, SERPINB5, THBS1 and TP53 gene (p < 0.05) and BCL2L2, CTSB, ITGB4, JUN, KIT, KLF5, SCGB1D2, SCGB2A1, SERPINE1 (p < 0.01), and EGFR, GABRP, GSN, MAP2K7 and THBS2 (p < 0.001), and GSN, KLK5 (p < 0.0001). The ontological gene analyses revealed high deregulations in gene group directly associated with breast cancer prognosis and origin.

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Acknowledgments

The authors thank Jana Guranova for her excellent technical support. This work was supported by Grant UK 243/2012 and VEGA 1/0243/12 from Ministry of Education.

Conflict of interest

The authors declare no conflict of interests.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Kollarova 2 Martin, 036 01, Bratislava, Slovak Republic, Slovakia

    Pavol Zubor, Petra Moricova, Ivana Kapustova, Andrea Mendelova & Jan Danko

  2. Department of Biochemistry, Jessenius Faculty of Medicine, Comenius University in Bratislava, Mala Hora 4 Martin, 036 01, Bratislava, Slovak Republic, Slovakia

    Jozef Hatok & Peter Racay

  3. Department of Pathology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Kollarova 2 Martin, 036 01, Bratislava, Slovak Republic, Slovakia

    Karol Kajo

  4. Department of Molecular Biology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Kollarova 2 Martin, 036 01, Bratislava, Slovak Republic, Slovakia

    Andrea Mendelova

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  1. Pavol Zubor
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  2. Jozef Hatok
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Correspondence to Pavol Zubor.

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Zubor, P., Hatok, J., Moricova, P. et al. Gene expression abnormalities in histologically normal breast epithelium from patients with luminal type of breast cancer. Mol Biol Rep 42, 977–988 (2015). https://doi.org/10.1007/s11033-014-3834-x

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  • DOI: https://doi.org/10.1007/s11033-014-3834-x

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