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Molecular cloning, expression profile, polymorphism and the genetic effects of the dopamine D1 receptor gene on duck reproductive traits

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Abstract

The dopamine D1 receptor (DRD1), a member of the dopamine receptor (DR) gene family, participates in the regulation of reproductive behaviors in birds. In this study, a 1,390 bp fragment covering the complete coding region (CDS) of duck DRD1 gene was obtained. The cDNA (GenBank: JQ346726) contains a 1,353 bp CDS and a 37 bp 3′- UTR including a TGA termination codon (nucleotides 1,354–1,356 bp). The duck DRD1 shares about 76–96 % nucleic acid identity and 82–98 % amino acid identity with their counterparts in other species. A phylogenetic tree based on amino acid sequences displays that duck DRD1 protein is closely related with those of chicken and zebra finch. The quantitative real-time PCR analysis indicates that the DRD1 mRNA is widely expressed in all examined tissues. Five single nucleotide polymorphisms (SNPs) (c.189A > T, c.507C > T, c.681C > T, c.765A > T, c.1044A > G) in the CDS of duck DRD1 gene were indentified, c.681C > T and c.765A > T were genotyped and analyzed in a two generations duck population by using of PCR–RFLP. Association analysis demonstrated that the c.681C > T genotypes were significantly associated with body weight at sexual maturity (when laying their first egg) (P < 0.01), egg production within 360 days (P < 0.05) and 420 days (P < 0.01); the c.765A > T genotypes were significantly associated with egg shape index and egg shell strength (P < 0.05). Those results suggest that the DRD1 gene may be a potential genetic marker to improve some reproductive traits in ducks.

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Acknowledgments

The authors thank Dr Xiangdong Liu and Ming Xiong for helping on the statistical analysis. This research was supported by the Project of Scientific Research and Development in Hubei Province (2011BBB096), the National Natural Science Foundation of China (31172201), Fundamental Research Funds for the Central Universities (2011QC006), and the Creative Team Project of Ministry of Education of China (IRT-0831).

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Authors and Affiliations

  1. Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Cui Wang, Shijun Li, Chuang Li, Yanping Feng, Xiuli Peng & Yanzhang Gong

  2. College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Yanzhang Gong

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  1. Cui Wang
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  2. Shijun Li
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  3. Chuang Li
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  4. Yanping Feng
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Correspondence to Yanzhang Gong.

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Wang, C., Li, S., Li, C. et al. Molecular cloning, expression profile, polymorphism and the genetic effects of the dopamine D1 receptor gene on duck reproductive traits. Mol Biol Rep 39, 9239–9246 (2012). https://doi.org/10.1007/s11033-012-1797-3

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  • DOI: https://doi.org/10.1007/s11033-012-1797-3

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