Molecular Biology Reports

, Volume 38, Issue 2, pp 893–903

Geographic distribution of phylogenetically-distinct legume pod borer, Maruca vitrata (Lepidoptera: Pyraloidea: Crambidae)

  • Venu M. Margam
  • Brad S. Coates
  • Malick N. Ba
  • Weilin Sun
  • Clementine L. Binso-Dabire
  • Ibrahim Baoua
  • Mohammad F. Ishiyaku
  • John T. Shukle
  • Richard L. Hellmich
  • Fernando G. Covas
  • Srinivasan Ramasamy
  • Joel Armstrong
  • Barry R. Pittendrigh
  • Larry L. Murdock
Article

DOI: 10.1007/s11033-010-0182-3

Cite this article as:
Margam, V.M., Coates, B.S., Ba, M.N. et al. Mol Biol Rep (2011) 38: 893. doi:10.1007/s11033-010-0182-3

Abstract

Maruca vitrata Fabricius is a pantropical lepidopteran pest of legumes. Phylogenetic analysis of a mitochondrial cytochrome c oxidase-I gene (cox1) fragment indicates that three Maruca sp. mitochondrial lineages have unique geographic distributions [lineages 1 and 2: Australia, Taiwan, and West Africa (Niger, Nigeria, and Burkina Faso), and lineage 3: Puerto Rico]. The haplotype (T30, T114) is specific to lineages 1&2 and was assayed by NsiI and SacI polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) within population samples; it was not observed in the Puerto Rican samples, but was nearly fixed among samples from West Africa, Australia and Taiwan (85.5–100%). Re-sequencing and phylogenetic analyses of PCR-RFLP defined cox1 haplotypes indicate that nucleotide diversity is highest among samples from West Africa. Phylogenetic reconstruction based upon ribosomal DNA (rDNA) internal transcribed spacer-2 (ITS-2) sequences provided additional evidence for three Maruca sp. clades. These data suggest that multiple unique Maruca species or subspecies are present worldwide, which has implications for the management of this pest species-complex.

Keywords

Legume pod borercox1BarcodingPopulation geneticsMaruca

Supplementary material

11033_2010_182_MOESM1_ESM.doc (28 kb)
Supplemental Fig. S1: Alignment of a 500 bp mitochondrial coxI fragment and corresponding translation obtained from Maruca sp. samples from Taiwan (TAI), Nigeria (NA), and Puerto Rico (PR). Only variable nucleotide positions are indicated, and non-synonymous changes in the peptide sequence are highlighted. The NsiI (ATGCAT) and SacI (GAGCTC) restriction endonuclease recognition sites that are diagnostic for differentiation of Maruca sp. from non-Puerto Rican locations are underlined. (DOC 28 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Venu M. Margam
    • 1
  • Brad S. Coates
    • 2
  • Malick N. Ba
    • 3
  • Weilin Sun
    • 4
  • Clementine L. Binso-Dabire
    • 3
  • Ibrahim Baoua
    • 5
  • Mohammad F. Ishiyaku
    • 6
  • John T. Shukle
    • 1
  • Richard L. Hellmich
    • 2
  • Fernando G. Covas
    • 7
  • Srinivasan Ramasamy
    • 8
  • Joel Armstrong
    • 9
  • Barry R. Pittendrigh
    • 4
  • Larry L. Murdock
    • 1
  1. 1.Department of EntomologyPurdue UniversityW LafayetteUSA
  2. 2.Corn Insects and Crop Genetics Research UnitUSDA–ARSAmesUSA
  3. 3.Institut de l’Environnement et de Recherches Agricoles (INERA)Ouagadougou 01Burkina Faso
  4. 4.Department of EntomologyUniversity of Illinois at Urbana-ChampaignChampaignUSA
  5. 5.Institut National de Recherche Agronomique du NigerMaradiNiger
  6. 6.Department of Plant Science, Institute for Agricultural ResearchAhmadu Bello UniversityZariaNigeria
  7. 7.University of Puerto RicoMayaguezUSA
  8. 8.AVRDC-The World Vegetable CenterTainanTaiwan
  9. 9.CSIRO EntomologyBlack MountainAustralia