Article

Molecular Biology Reports

, Volume 38, Issue 2, pp 893-903

First online:

Geographic distribution of phylogenetically-distinct legume pod borer, Maruca vitrata (Lepidoptera: Pyraloidea: Crambidae)

  • Venu M. MargamAffiliated withDepartment of Entomology, Purdue University Email author 
  • , Brad S. CoatesAffiliated withCorn Insects and Crop Genetics Research Unit, USDA–ARS
  • , Malick N. BaAffiliated withInstitut de l’Environnement et de Recherches Agricoles (INERA)
  • , Weilin SunAffiliated withDepartment of Entomology, University of Illinois at Urbana-Champaign
  • , Clementine L. Binso-DabireAffiliated withInstitut de l’Environnement et de Recherches Agricoles (INERA)
  • , Ibrahim BaouaAffiliated withInstitut National de Recherche Agronomique du Niger
  • , Mohammad F. IshiyakuAffiliated withDepartment of Plant Science, Institute for Agricultural Research, Ahmadu Bello University
  • , John T. ShukleAffiliated withDepartment of Entomology, Purdue University
  • , Richard L. HellmichAffiliated withCorn Insects and Crop Genetics Research Unit, USDA–ARS
    • , Fernando G. CovasAffiliated withUniversity of Puerto Rico
    • , Srinivasan RamasamyAffiliated withAVRDC-The World Vegetable Center
    • , Joel ArmstrongAffiliated withCSIRO Entomology
    • , Barry R. PittendrighAffiliated withDepartment of Entomology, University of Illinois at Urbana-Champaign
    • , Larry L. MurdockAffiliated withDepartment of Entomology, Purdue University

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Abstract

Maruca vitrata Fabricius is a pantropical lepidopteran pest of legumes. Phylogenetic analysis of a mitochondrial cytochrome c oxidase-I gene (cox1) fragment indicates that three Maruca sp. mitochondrial lineages have unique geographic distributions [lineages 1 and 2: Australia, Taiwan, and West Africa (Niger, Nigeria, and Burkina Faso), and lineage 3: Puerto Rico]. The haplotype (T30, T114) is specific to lineages 1&2 and was assayed by NsiI and SacI polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) within population samples; it was not observed in the Puerto Rican samples, but was nearly fixed among samples from West Africa, Australia and Taiwan (85.5–100%). Re-sequencing and phylogenetic analyses of PCR-RFLP defined cox1 haplotypes indicate that nucleotide diversity is highest among samples from West Africa. Phylogenetic reconstruction based upon ribosomal DNA (rDNA) internal transcribed spacer-2 (ITS-2) sequences provided additional evidence for three Maruca sp. clades. These data suggest that multiple unique Maruca species or subspecies are present worldwide, which has implications for the management of this pest species-complex.

Keywords

Legume pod borer cox1 Barcoding Population genetics Maruca