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miR-27b targets LIMK1 to inhibit growth and invasion of NSCLC cells

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Abstract

Non-small cell lung cancer (NSCLC), which accounts for ~80 % of lung cancer cases, is one of the most common causes for cancer-related death. microRNAs (miRNAs) have been found to play critical roles in the development and progression of NSCLC. miR-27b has recently been reported as a tumor suppressor in several cancers, but its role in NSCLC remains poorly understood. In this study, we found that miR-27b was remarkably decreased in both NSCLC tissues and cell lines. Moreover, overexpression of miR-27b significantly suppressed NSCLC cells proliferation and invasion. LIM kinase 1 (LIMK1), an essential protein for malignant transformation, was found to be a target of miR-27b. Ectopic expression of LIMK1 dramatically dampened mir-27b action of cancer inhibition. Finally, LIMK1 was found to be negatively correlated with miR-27b in NSCLC patients. Our results demonstrated a tumor-suppressive role of miR-27b in NSCLC, suggesting a potential therapeutic target for NSCLC.

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We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Li Wan, Lin Zhang, Kai Fan & Jianjun Wang

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  1. Li Wan
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  2. Lin Zhang
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  3. Kai Fan
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  4. Jianjun Wang
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Correspondence to Jianjun Wang.

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Wan, L., Zhang, L., Fan, K. et al. miR-27b targets LIMK1 to inhibit growth and invasion of NSCLC cells. Mol Cell Biochem 390, 85–91 (2014). https://doi.org/10.1007/s11010-013-1959-1

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  • DOI: https://doi.org/10.1007/s11010-013-1959-1

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