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Nitrite, a Reactive Nitrogen Species, Protects Human Alpha-2-Macroglobulin from Halogenated Oxidant, HOCl

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Abstract

Reactive nitrogen species have been implicated in the pathogenesis of over 40 human diseases, including inflammation. Evidences suggest that reactive nitrogen species such as nitrite/nitrate and halogenated oxidant-HOCl accumulate at the site of inflammation. At physiologically attainable concentrations, HOCl was found to significantly damage the antiproteolytic potential of human α2M and induce subtle changes in conformation as judged by fluorescence analysis. Our studies further suggest that at physiological concentrations, nitrite offered significant protection against HOCl induced α2M inactivation. Our studies suggest that nitrite may act as an antioxidant at physiological concentrations by removing HOCl at sites where both NO2 and HOCl are formed.

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Abbreviations

α2M:

Alpha-2-Macroglobulin

HOCl:

Hypochlorous acid

NO2 :

Nitrite

NO2 − :

Nitrite anion

NO:

Nitric oxide

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Acknowledgments

MWK would like to thank the Indian Council of Medical Research, New Delhi for a research fellowship. FHK acknowledges the generous financial support provided by UGC-DRS program for this study.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, India

    M. Wasim Khan, Ashreeb Naqshbandi, Haseeb Zubair & Fahim H. Khan

  2. Department of Biochemistry, Faculty of Dentistry, Jamia Millia Islamia, New Delhi, 110025, India

    Haseeb Ahsan

  3. Department of Medicine, Division of Biological Sciences, Section of Emergency Medicine, The University of Chicago, Chicago, IL, 60637, USA

    Shakil A. Khan

Authors
  1. M. Wasim Khan
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  2. Ashreeb Naqshbandi
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  3. Haseeb Zubair
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  4. Haseeb Ahsan
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  5. Shakil A. Khan
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  6. Fahim H. Khan
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Correspondence to Fahim H. Khan.

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Wasim Khan, M., Naqshbandi, A., Zubair, H. et al. Nitrite, a Reactive Nitrogen Species, Protects Human Alpha-2-Macroglobulin from Halogenated Oxidant, HOCl. Protein J 29, 276–282 (2010). https://doi.org/10.1007/s10930-010-9249-1

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