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TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins

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Abstract

A TROSY-selected ZZ-exchange experiment is described for measuring slow chemical exchange rates by monitoring the TROSY component of 15N longitudinal magnetization. Application of the proposed pulse sequence to the cadherin 8 N-terminal extracelluar domain demonstrates that enhanced sensitivity is obtained, compared to a previously described TROSY-detected ZZ-exchange sequence (Sahu et al. J Am Chem Soc 129: 13232–13237, 2007), by preserving the TROSY effect during the mixing period as well as the frequency encoding periods.

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Acknowledgments

This work was supported by National Institutes of Health (NIH) grant GM59273 (A. G. P.). We thank Fabiana Bahna and Prof. Lawrence Shapiro for assistance in sample preparation.

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  1. Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY, 10032, USA

    Ying Li & Arthur G. Palmer III

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  1. Ying Li
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  2. Arthur G. Palmer III
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Correspondence to Arthur G. Palmer III.

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Li, Y., Palmer, A.G. TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins. J Biomol NMR 45, 357–360 (2009). https://doi.org/10.1007/s10858-009-9385-0

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  • DOI: https://doi.org/10.1007/s10858-009-9385-0

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