In vitro maturation of oocytes via the pre-fabricated self-assembled artificial human ovary
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Create a 3-Dimensional artificial human ovary to mature human oocytes.
Theca and granulosa cells were isolated from antral follicles of reproductive-aged women, seeded into micro-molded gels and self-assembled into complex 3D microtissues. Immunohistochemistry and live-dead staining confirmed theca cell identity and cellular viability at one week respectively. Placement of granulosa cell spheroids or cumulus-oocyte complexes into theca cell honeycomb openings resulted in creation of an artificial human ovary. Oocytes from this construct were assessed for polar body extrusion.
Theca and granulosa cells self-assembled into complex microtissues, remaining viable for one week. At 72 h after artificial human ovary construction, theca cells completely surrounded the granulosa spheroids or COCs without stromal invasion or disruption. Polar body extrusion occurred in one of three COCs assessed.
An artifical human ovary can be created with self-assembled human theca and granulosa cell microtissues, and used for IVM and future oocyte toxicology studies.
- In vitro maturation of oocytes via the pre-fabricated self-assembled artificial human ovary
Journal of Assisted Reproduction and Genetics
Volume 27, Issue 12 , pp 743-750
- Cover Date
- Print ISSN
- Online ISSN
- Springer US
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- Artificial ovary
- In vitro maturation
- Theca cells
- Tissue engineering
- Industry Sectors
- Author Affiliations
- 1. Division of Reproductive Endocrinology & Infertility, Department of Obstetrics & Gynecology, Women & Infants’ Hospital, Alpert Medical School, Brown University, Providence, RI, 02905, USA
- 4. Department of Molecular Pharmacology, Physiology & Biotechnology, Center for Biomedical Engineering, Brown University, Providence, RI, 02912, USA
- 2. Program in Women’s Oncology, Department of Obstetrics & Gynecology, Women & Infants’ Hospital, Alpert Medical School, Brown University, Providence, RI, 02905, USA
- 3. Department of Pathology, Women & Infants’ Hospital, Alpert Medical School, Brown University, Providence, RI, 02905, USA