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Specific binding of tubeimoside-2 with proteins in hepatocarcinoma HepG2 cells: investigation by molecular spectroscopy

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Journal of Applied Spectroscopy Aims and scope

In this study, we compared different binding interactions of TBMS2 with proteins both in hepatocarcinoma HepG2 cells and in normal embryo hepatic L02 cells by using fluorescence, absorption, and CD spectroscopy. The fluorescence data revealed that the fluorescence intensity of proteins in the HepG2 and L02 cells decreased in the presence of TBMS2 by 30.79% and 12.01%, respectively. Binding constants and thermodynamic parameters were obtained for systems of TBMS2 with the two kinds of cell proteins. The results indicated that HepG2 cell proteins had a higher TBMS2 binding activity than those in the L02 cells. Analysis of the TBMS2 cytotoxic activities showed that TBMS2 could selectively induce apoptosis of HepG2 cells by binding to them, while its apoptotic effect on L02 cells was relatively weaker.

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Authors and Affiliations

  1. School of Chemical Engineering, Northwest University, Xi’an, Shaanxi, 710069, China

    Sun Yang & Fan Jun

  2. Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi’an, Shaanxi, China

    Sun Shi-Sheng

  3. Biomedicine Key Laboratory of Shaanxi Province, Northwest University, Xi’an, China

    Zhao Ying-Yong

Authors
  1. Sun Yang
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  2. Sun Shi-Sheng
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  3. Zhao Ying-Yong
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  4. Fan Jun
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Correspondence to Fan Jun.

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Published in Zhurnal Prikladnoi Spektroskopii, Vol. 79, No. 3, pp. 436–442, May–June, 2012

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Yang, S., Shi-Sheng, S., Ying-Yong, Z. et al. Specific binding of tubeimoside-2 with proteins in hepatocarcinoma HepG2 cells: investigation by molecular spectroscopy. J Appl Spectrosc 79, 416–423 (2012). https://doi.org/10.1007/s10812-012-9617-z

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  • DOI: https://doi.org/10.1007/s10812-012-9617-z

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