Chlorophyll Revisited: Anti-inflammatory Activities of Chlorophyll a and Inhibition of Expression of TNF-α Gene by the Same
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In view of the folklore use of green leaves to treat inflammation, the anti-inflammatory property of chlorophylls and their degradation products were studied. Chlorophyll a and pheophytin a (magnesium-free chlorophyll a) from fresh leaves showed potent anti-inflammatory activity against carrageenan-induced paw edema in mice and formalin-induced paw edema in rats. Chlorophyll a inhibited bacterial lipopolysaccharide-induced TNF-α (a pro-inflammatory cytokine) gene expression in HEK293 cells, but it did not influence the expression of inducible nitric acid synthase and cyclooxygenase-2 genes. Chlorophyll b only marginally inhibited both inflammation and TNF-α gene expression. But both chlorophyll a and chlorophyll b showed the same level of marginal inhibition on 12-O-tetradecanoyl-phorbol-13-acetate-induced NF-κB activation. Chlorophylls and pheophytins showed in vitro anti-oxidant activity. The study shows that chlorophyll a and its degradation products are valuable and abundantly available anti-inflammatory agents and promising for the development of phytomedicine or conventional medicine to treat inflammation and related diseases.
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- Chlorophyll Revisited: Anti-inflammatory Activities of Chlorophyll a and Inhibition of Expression of TNF-α Gene by the Same
Volume 35, Issue 3 , pp 959-966
- Cover Date
- Print ISSN
- Online ISSN
- Springer US
- Additional Links
- pheophytin a
- paw edema
- nuclear factor-κB
- Streblus asper Lour.
- Chromolaena odorata (L.) R. King & Robin
- Industry Sectors
- Author Affiliations
- 1. Phytochemistry and Phytopharmacology Division, Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram District, Pin: 695562, India
- 2. Rajiv Gandhi Centre for Biotechnology, Poojapura, Thiruvananthapuram, India
- 3. Health and Research Centre, Kumarapuram, Thiruvananthapuram, India
- 4. Department of Biotechnology, Indian Institute of Technology Madras, Chennai, India