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Moringa oleifera Pod Inhibits Inflammatory Mediator Production by Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage Cell Lines

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Abstract

Pro-inflammatory mediators produced during inflammatory response have been demonstrated to initiate and aggravate pathological development of several chronic diseases. Plant bioactive constituents have been reported to exert anti-inflammatory activities. Various parts of Moringa oleifera have long been used as habitual diets and traditional remedy along the tropical region. Anti-inflammatory activity of boiled M. oleifera pod extract was assessed by measuring pro-inflammatory mediator expression in the lipopolysaccharide-induced murine RAW264.7 macrophage cells. Prior treatment with 31–250 μg/mL M. oleifera extract for 1 h inhibited elevation of mRNA and protein level of interleukine-6, tumor necrosis factor-alpha, inducible nitric oxide synthase, and cyclooxygenease-2, induced by lipopolysaccharide for 24 h in a dose-dependent manner. The suppressive effect was mediated partly by inhibiting phosphorylation of inhibitor kappa B protein and mitogen-activated protein kinases. These results indicate that the anti-inflammatory activity from bioactive compounds present in the M. oleifera pod constituents may contribute to ameliorate the pathogenesis of inflammatory-associated chronic diseases.

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Acknowledgment

This study was supported by National Science and Technology Development Agency, Thailand. The authors thank Dr. Harold C. Furr for his helpful commentary during manuscript preparation.

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  1. Institute of Nutrition, Mahidol University, Putthamonthon 4 Rd, Nakhon Pathom, 73170, Thailand

    Channarong Muangnoi, Pimjai Chingsuwanrote, Phawachaya Praengamthanachoti & Siriporn Tuntipopipat

  2. Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Putthamonthon 4 Rd, Nakhon Pathom, 73170, Thailand

    Saovaros Svasti

  3. Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand

    Saovaros Svasti

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  1. Channarong Muangnoi
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Correspondence to Siriporn Tuntipopipat.

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Muangnoi, C., Chingsuwanrote, P., Praengamthanachoti, P. et al. Moringa oleifera Pod Inhibits Inflammatory Mediator Production by Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage Cell Lines. Inflammation 35, 445–455 (2012). https://doi.org/10.1007/s10753-011-9334-4

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