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Stock-plant etiolation causes drifts in total soluble sugars and anthraquinones, and promotes adventitious root formation in teak (Tectona grandis L. f.) coppice shoots

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Abstract

The effects of stock-plant etiolation on coppice-shoot growth, drifts in total soluble sugars and anthraquinones (AQs; C14H8O2), and rooting potentiality of shoot cuttings were examined in Tectona grandis L. f. (clone FG1). When seedlings were one-year-old, they were coppiced and maintained in the dark for etiolation, with a parallel set kept under natural light in an open environment. Coppice shoots were made into single-node leafy cuttings (SNCs), which were cultured under intermittent mist for rooting. These SNCs were treated with different concentrations of NAA (0, 2000 and 3000 mg l−1). Etiolation significantly increased the coppice-shoot length, internode length, number of coppice shoots, number of leaves, number of nodes and total soluble sugars. The HPTLC analysis showed qualitative and quantitative differences in AQs in coppice shoots obtained from etiolated and non-etiolated stock plants. The study showed that AQs could be used as a marker for maturity and juvenility in teak. Stock-plant etiolation caused a significant increase in percent rooting and sprouting, shoot length, number of shoots and number of leaves per SNC, but a decrease in callusing at the base of the SNC. NAA at 2000 and 3000 mg l−1 had inhibitory effects on rooting and sprouting of SNCs. The result showed that stock-plant etiolation fostered rooting by rejuvenating the coppice shoots.

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Fig. 1
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Abbreviations

AQ:

Anthraquinone

NAA:

α-naphthaleneacetic acid

HPTLC:

High performance thin layer chromatography

SNC:

Single-node leafy coppice-shoot cutting

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Acknowledgments

This work was supported by the World Bank FREE Projects and Indian Council of Forestry Research and Education, Dehra Dun, India. I thank Director and his team (Dr. S. Mahrotra and Dr. Sayyada Khatoon) of National Botanical Research Institute (NBRI), Lucknow, India for providing facilities for HPTLC work.

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  1. Azamal Husen

    Present address: Department of Applied Biology, Faculty of Applied Natural Sciences, University of Gondar, P.O. Box 1326, Gondar, Ethiopia

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  1. Plant Physiology, Botany Division, Forest Research Institute, P.O. New Forest, Dehra Dun, 248006, India

    Azamal Husen

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Correspondence to Azamal Husen.

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Husen, A. Stock-plant etiolation causes drifts in total soluble sugars and anthraquinones, and promotes adventitious root formation in teak (Tectona grandis L. f.) coppice shoots. Plant Growth Regul 54, 13–21 (2008). https://doi.org/10.1007/s10725-007-9222-y

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