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Assessment of Genetic Diversity among Finger Millet (Eleusine coracana (L.) Gaertn.) Accessions using Molecular Markers

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Abstract

Finger millet (Eleusine coracana), an allotetraploid cereal, is widely cultivated in the arid and semiarid regions of the world. Being rich in protein and calcium, finger millet serves as an important staple food for rural populations in developing tropical countries where calcium deficiency and anemia are wide spread. Thirty-two finger millet genotypes were fingerprinted using 50 random amplified polymorphic DNA (RAPD) markers. Out of the total 529 loci generated using the 50 RAPD primers, 479 loci (91%) were polymorphic and informative to differentiate the accessions. Cluster analysis grouped the 32 finger millet accessions into two major clusters. Among the 32 finger millet genotypes, GEC 182 and CO 12 were distantly related with a low similarity index of 0.315. These two accessions also differed considerably in days to flowering and grain weight; GEC 182 is early flowering and has bold grains, while CO 12 is late flowering and has smaller grains. These two accessions with higher diversity at molecular level, phenology and grain weight will be ideal as parents in hybridization programme, to develop improved finger millet varieties suitable for peninsular region of India.

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Abbreviations

AFLP:

Amplified Fragment Length Polymorphism

RAPD:

Random Amplified Polymorphic DNA

RFLP:

Restriction Fragment Length Polymorphism

SAHN:

Sequential Agglomerative Hierarchial Non overlapping

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Authors and Affiliations

  1. Department of Plant Molecular Biology and Biotechnology, Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, 641003, Coimbatore, India

    B. Kalyana Babu, S. Michael Gomez, K. R. Biji, N. S. Rajendraprasad, S. Satheesh Kumar & R. Chandra Babu

  2. Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, 641003, Coimbatore, India

    N. Senthil

Authors
  1. B. Kalyana Babu
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  2. N. Senthil
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  3. S. Michael Gomez
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  4. K. R. Biji
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  5. N. S. Rajendraprasad
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  6. S. Satheesh Kumar
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  7. R. Chandra Babu
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Correspondence to R. Chandra Babu.

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Babu, B.K., Senthil, N., Gomez, S.M. et al. Assessment of Genetic Diversity among Finger Millet (Eleusine coracana (L.) Gaertn.) Accessions using Molecular Markers. Genet Resour Crop Evol 54, 399–404 (2007). https://doi.org/10.1007/s10722-006-0002-8

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  • DOI: https://doi.org/10.1007/s10722-006-0002-8

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