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RAPD and ISSR molecular markers in Olea europaea L.: Genetic variability and molecular cultivar identification

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Abstract

Thirty Portuguese and eight foreign olive (Olea europaea L.) cultivars were screened using Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) markers. Twenty RAPD primers amplified 301 reproducible bands of which 262 were polymorphic; and 17 ISSR primers amplified 204 bands of which 180 were polymorphic. The percentage of polymorphic bands detected by ISSR and RAPD was similar (88 and 87%, respectively). The genetic variability observed was similar in the Portuguese and foreign olive cultivars. Seven ISSR and 12 RAPD primers were able to distinguish individually all 38 olive cultivars. Twenty specific molecular markers are now available to be converted into Sequence Characterised Amplified Region (SCAR) markers. Relationships among Portuguese and foreign cultivars is discussed.

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Authors and Affiliations

  1. Department of Genetics and Biotechnology, Centre of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, P.O. Box 1013, 5000-911, Vila Real, Portugal

    Paula Martins-Lopes, José Lima-Brito, Sónia Gomes, Julieta Meirinhos & Henrique Guedes-Pinto

  2. Department of Oliviculture, National Plant Breeding Station, INIA, P.O. Box 6, 7350-951, Elvas, Portugal

    Luís Santos

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  1. Paula Martins-Lopes
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  2. José Lima-Brito
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  3. Sónia Gomes
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  4. Julieta Meirinhos
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  5. Luís Santos
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  6. Henrique Guedes-Pinto
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Correspondence to Henrique Guedes-Pinto.

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Martins-Lopes, P., Lima-Brito, J., Gomes, S. et al. RAPD and ISSR molecular markers in Olea europaea L.: Genetic variability and molecular cultivar identification. Genet Resour Crop Evol 54, 117–128 (2007). https://doi.org/10.1007/s10722-005-2640-7

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  • DOI: https://doi.org/10.1007/s10722-005-2640-7

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