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Haemato-immunology and histo-architectural changes in Labeo rohita fingerlings: effect of dietary aflatoxin and mould inhibitor

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Abstract

A feeding trail of 60 days was conducted for delineating the effect of dietary aflatoxin (AFB1) with or without supplementation of a mixture of mould inhibitor (0.25% clove oil + 0.32% sodium propionate) on haematology, respiratory burst activity and histology of Labeo rohita fingerlings. Three hundred and sixty fishes (avg. wt. 1.48–1.54 g) were randomly distributed into eight treatment groups. Eight experimental diets with four different levels of aflatoxin (0, 10, 20 and 40 ppb) with or without mould inhibitor were prepared. Haematological parameters like total serum protein, albumin, globulin and A:G ratio were significantly (P < 0.05) reduced with increasing levels of aflatoxin in the diet. However, supplementation of mould inhibitor showed enhanced values when compared to non-supplemented counter parts suggesting ameliorating effects of mould inhibitor on aflatoxin. Total leucocyte count was higher in aflatoxin-treated groups. Histological observations were complementary to haematological parameters. Respiratory burst activity was significantly (P < 0.05) decreased in higher aflatoxin-treated groups but not affected significantly (P > 0.05) due to inclusion of inhibitor alone and/or interaction of aflatoxin level and inhibitor in the diet. From this study, it was concluded that up to 20 ppb aflatoxin level in the diet the haemato-immunological parameters are protected.

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Acknowledgments

The authors are grateful to The Director, Central Institute of Fisheries Education, Mumbai, for providing facilities for carrying out the research work. The first author is grateful to Central Institute of Fisheries Education, Mumbai, for financial support by awarding Institutional Fellowship.

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Authors and Affiliations

  1. Department of Aquaculture, Central Institute of Fisheries Education, Versova, Mumbai, 400061, India

    S. Mohapatra & A. K. Prusty

  2. Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai, 400061, India

    N. P. Sahu, A. K. Pal & Shivendra Kumar

  3. Department of Aquaculture System and Animal Nutrition in the Tropics and Subtropics (480b), University of Hohenheim, 70599, Stuttgart, Germany

    Vikas Kumar

  4. Krishi Vigyan Kendra, Rajendra Agricultural University, Madhopur, 845454, W. Champaran, Bihar, India

    Shivendra Kumar

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  1. S. Mohapatra
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Correspondence to Shivendra Kumar.

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Mohapatra, S., Sahu, N.P., Pal, A.K. et al. Haemato-immunology and histo-architectural changes in Labeo rohita fingerlings: effect of dietary aflatoxin and mould inhibitor. Fish Physiol Biochem 37, 177–186 (2011). https://doi.org/10.1007/s10695-010-9428-1

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  • DOI: https://doi.org/10.1007/s10695-010-9428-1

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