Skip to main content

Advertisement

SpringerLink
Log in

A functional HOTAIR rs920778 polymorphism does not contributes to gastric cancer in a Turkish population: a case–control study

  • Original Article
  • Published:
Familial Cancer Aims and scope Submit manuscript

Abstract

An aberrant up-regulation of HOX transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), is associated with human cancers including gastric cancer (GC) and worse clinicopathological features. A naturally occurring functional single nucleotide polymorphism (SNP) rs920,778 (C→T) in the intronic enhancer of HOTAIR gene has been demonstrated to affect HOTAIR expression and cancer susceptibility. To investigate the association of the HOTAIR rs920778 polymorphism on the risk of GC susceptibility in Turkish population, a hospital-based case–control study was carried out consisting of 104 GC and 209 healthy control subjects matched on age and gender. The genotype frequency of HOTAIR rs920778 polymorphism was determined by using TaqMan Real-Time Polymerase Chain Reaction. No statistically significant differences were found in the allele or genotype distributions of the HOTAIR rs920778 polymorphism among GC and healthy control subjects (P > 0.05). Our results demonstrate that the HOTAIR rs920778 polymorphism has not been in any major role in genetic susceptibility to gastric carcinogenesis, at least in the population studied here. Independent studies are needed to validate our findings in a larger series, as well as in patients of different ethnic origins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray F (2014) Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. doi:10.1002/ijc.29210

    PubMed  Google Scholar 

  2. Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray, F. GLOBOCAN 2012 v1.0, Cancer incidence and mortality worldwide: IARC Cancer Base No. 11 [Internet]. Lyon, France: International Agency for Research on Cancer; 2013. Available from: http://globocan.iarc.fr. Accessed on 22 Jan 2015

  3. Shi J, Qu YP, Hou P (2014) Pathogenetic mechanisms in gastric cancer. World J Gastroenterol 20:13804–13819. doi:10.3748/wjg.v20.i38.13804

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Resende C, Thiel A, Machado JC, Ristimäki A (2011) Gastric cancer: basic aspects. Helicobacter 16(Suppl 1):38–44. doi:10.1111/j.1523-5378.2012.00979.x

    Article  CAS  PubMed  Google Scholar 

  5. Ludwig JA, Weinstein JN (2005) Biomarkers in cancer staging, prognosis and treatment selection. Nat Rev Cancer 5:845–856. doi:10.1038/nrc1739

    Article  CAS  PubMed  Google Scholar 

  6. Rinn JL, Kertesz M, Wang JK, Squazzo SL, Xu X, Brugmann SA, Goodnough LH, Helms JA, Farnham PJ, Segal E, Chang HY (2007) Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs. Cell 129:1311–1323. doi:10.1016/j.cell.2007.05.022

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Zhang J, Zhang P, Wang L, Piao HL, Ma L (2014) Long non-coding RNA HOTAIR in carcinogenesis and metastasis. Acta Biochim Biophys Sin (Shanghai) 46:1–5. doi:10.1093/abbs/gmt117

    Article  Google Scholar 

  8. Zhang S, Chen S, Yang G, Gu F, Li M, Zhong B, Hu J, Hoffman A, Chen M (2014) Long noncoding RNA HOTAIR as an independent prognostic marker in cancer: a meta-analysis. PLoS One 9:e105538. doi:10.1371/journal.pone.0105538

    Article  PubMed Central  PubMed  Google Scholar 

  9. Wu Y, Zhang L, Wang Y, Li H, Ren X, Wei F, Yu W, Wang X, Zhang L, Yu J, Hao X (2014) Long noncoding RNA HOTAIR involvement in cancer. Tumour Biol 35:9531–9538. doi:10.1007/s13277-014-2523-7

    Article  CAS  PubMed  Google Scholar 

  10. Lee NK, Lee JH, Park CH, Yu D, Lee YC, Cheong JH, Noh SH, Lee SK (2014) Long non-coding RNA HOTAIR promotes carcinogenesis and invasion of gastric adenocarcinoma. Biochem Biophys Res Commun 451:171–178. doi:10.1016/j.bbrc.2014.07.067

    Article  CAS  PubMed  Google Scholar 

  11. Xu ZY, Yu QM, Du YA, Yang LT, Dong RZ, Huang L, Yu PF, Cheng XD (2013) Knockdown of long non-coding RNA HOTAIR suppresses tumor invasion and reverses epithelial-mesenchymal transition in gastric cancer. Int J Biol Sci 9:587–597. doi:10.7150/ijbs.6339

    Article  PubMed Central  PubMed  Google Scholar 

  12. Endo H, Shiroki T, Nakagawa T, Yokoyama M, Tamai K, Yamanami H, Fujiya T, Sato I, Yamaguchi K, Tanaka N, Iijima K, Shimosegawa T, Sugamura K, Satoh K (2013) Enhanced expression of long non-coding RNA HOTAIR is associated with the development of gastric cancer. PLoS One 8:e77070. doi:10.1371/journal.pone.0077070

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  13. Hajjari M, Behmanesh M, Sadeghizadeh M, Zeinoddini M (2013) Up-regulation of HOTAIR long non-coding RNA in human gastric adenocarcinoma tissues. Med Oncol 30:670. doi:10.1007/s12032-013-0670-0

    Article  PubMed  Google Scholar 

  14. Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ, Tsai MC, Hung T, Argani P, Rinn JL, Wang Y, Brzoska P, Kong B, Li R, West RB, van de Vijver MJ, Sukumar S, Chang HY (2010) Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature 464:1071–1076. doi:10.1038/nature08975

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  15. Zhang X, Zhou L, Fu G, Sun F, Shi J, Wei J, Lu C, Zhou C, Yuan Q, Yang M (2014) The identification of an ESCC susceptibility SNP rs920778 that regulates the expression of lncRNA HOTAIR via a novel intronic enhancer. Carcinogenesis 35:2062–2067. doi:10.1093/carcin/bgu103

    Article  CAS  PubMed  Google Scholar 

  16. Bayram S, Sümbül AT, Batmacı CY, Genç A (2015) Effect of HOTAIR rs920778 polymorphism on breast cancer susceptibility and clinicopathologic features in a Turkish population. Tumour Biol. doi:10.1007/s13277-014-3028-0

    Google Scholar 

  17. Pan W, Liu L, Wei J, Ge Y, Zhang J, Chen H, Zhou L, Yuan Q, Zhou C, Yang M (2015) A functional lncRNA HOTAIR genetic variant contributes to gastric cancer susceptibility. Mol Carcinog. doi:10.1002/mc.22261

    Google Scholar 

  18. Gauderman WJ, Morrison JM. Quanto 1.1: A computer program for power and sample size calculations for genetic-epidemiology studies, http://hydra.usc.edu/gxe, 2006

  19. Rodriguez S, Gaunt TR, Day IN (2009) Hardy–Weinberg equilibrium testing of biological ascertainment for Mendelian randomization studies. Am J Epidemiol 169(4):505–514. doi:10.1093/aje/kwn359

    Article  PubMed Central  PubMed  Google Scholar 

  20. Solé X, Guinó E, Valls J, Iniesta R, Moreno V (2006) SNPStats: a web tool for the analysis of association studies. Bioinformatics 22(15):1928–1929. doi:10.1093/bioinformatics/btl268

    Article  PubMed  Google Scholar 

  21. Guo W, Dong Z, Bai Y, Guo Y, Shen S, Kuang G, Xu J (2014) Associations between polymorphisms of HOTAIR and risk of gastric cardia adenocarcinoma in a population of north China. Tumour Biol. doi:10.1007/s13277-014-2912-y

    Google Scholar 

Download references

Acknowledgments

The author thanks all the subjects who participated in this study. The authors also would like to thank all Adıyaman University Scientific Research Unit staffs (Sel, A.; İlgin, K.; Elbir, F.; Işik, Y.; Abaci, U.). We also would like to thank Dr. Muhsin Aydın for very helpful English corrections. This work was supported by Adıyaman University Research Fund SYOBAP/2013-0001.

Conflict of interest

All of the authors declare that there are no conflicts of interest.

Author information

Authors and Affiliations

  1. Department of Nursing, Adıyaman School of Health, Adıyaman University, 02040, Adıyaman, Turkey

    Süleyman Bayram

  2. Department of Gastroenterology, Education and Research Hospital, Adıyaman University, 02040, Adıyaman, Turkey

    Yakup Ülger

  3. Department of Medical Oncology, Medical Faculty, Mustafa Kemal University, 31000, Hatay, Turkey

    Ahmet Taner Sümbül

  4. Department of Gastroenterology, Isparta State Hospital, 32100, Isparta, Turkey

    Berrin Yalınbaş Kaya

  5. Department of General Surgery, Medical Faculty, Çukurova University, 01330, Adana, Turkey

    Ahmet Rencüzoğulları

  6. Vocational School of Health Services, Adıyaman Univesity, 02040, Adıyaman, Turkey

    Ahmet Genç

  7. Department of Biology, Faculty of Science and Letters, Adıyaman University, 02040, Adıyaman, Turkey

    Yusuf Sevgiler & Eyyüp Rencüzoğulları

  8. Department of Biology, Institute of Science, Adıyaman University, 02040, Adıyaman, Turkey

    Onur Bozkurt

Authors
  1. Süleyman Bayram
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yakup Ülger
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ahmet Taner Sümbül
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Berrin Yalınbaş Kaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ahmet Rencüzoğulları
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ahmet Genç
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yusuf Sevgiler
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Onur Bozkurt
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Eyyüp Rencüzoğulları
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Süleyman Bayram.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bayram, S., Ülger, Y., Sümbül, A.T. et al. A functional HOTAIR rs920778 polymorphism does not contributes to gastric cancer in a Turkish population: a case–control study. Familial Cancer 14, 561–567 (2015). https://doi.org/10.1007/s10689-015-9813-0

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10689-015-9813-0

Keywords

Search

Navigation