Abstract
Wheat breeding programs worldwide aim at developing cultivars that meet end user quality attributes demanded by producers, processors, and consumers. Selecting from breeding populations created from well characterized parental germplasm provides the best opportunity of identifying cultivars that combine the best alleles and grain phenotypes for the desired technological applications. In this study, 216 bread wheat lines associated with Ethiopian and Kenyan breeding programs including a few founder lines were profiled for high molecular weight glutenin subunits and low molecular weight glutenin subunits by SDS-PAGE. Additionally, total crude protein, relative puroindoline content by SDS-PAGE, kernel diameter, kernel weight and kernel hardness by SKCS were determined. Extensive allelic variation at the glutenin subunit loci was found, with Glu-B1 and Glu-B3 having highest diversity across subpopulations. Relative to wild type cultivar ‘Alpowa-1-soft’, the founder line BW21 had the lowest puroindoline content. The frequencies of soft and very soft wheat classes were lowest in the Ethiopian subpopulation and highest among the Kenyan lines. Accordingly, 12 lines considered to have optimal combinations of glutenin subunit alleles and kernel characteristics were highlighted and recommended for cultivar improvement.
Similar content being viewed by others
References
AACC International (2010) Approved methods of analysis, 11th edn. AACC International, St. Paul Available online only
Allard RW (1960) Principles of plant breeding. Wiley, Ney York
Bernardo R (2003) Parental selection, number of breeding populations, and size of each population in inbred development. Theor Appl Genet 107:1252–1256
Branlard G, Dardevet M, Saccomano R, Lagoutte F, Gourdon J (2001) Genetic diversity of wheat storage proteins and bread wheat quality. Euphytica 119:57–67
Brites CM, Dos Santos CAL, Bagulho AS, Beirão-da-Costa M (2008) Effect of wheat puroindoline alleles on functional properties of starch. Eur Food Res Technol 226:1205–1212
Caballero L, Martin LM, Alvarez JB (2004) Variation and genetic diversity for gliadins in Spanish spelt wheat accessions. Genetic Res Crop Evol 51:679–686
Curtis BC, Rajaram S, Macpherson HG (2002) Bread wheat improvement and production. FAO, Rome
Dessalegn T, Van Deventer CS, Labuschagne MT, Martens H (2011) Allelic variation of HMW glutenin subunits of Ethiopian bread wheat cultivars and their quality. Afr Crop Sci J 19:55–63
Dixon GE (1960) A review of wheat breeding in Kenya. Euphytica 9:209–221
D’Ovidio R, Masci S (2004) The low-molecular-weight glutenin subunits of wheat gluten. J Cereal Sci 39:321–339
Dubin HJ, Brennan JP (2009) Combating stem and leaf rust of wheat: historical perspective, impacts, and lessons learned. International Food Policy Research Institute (IFPRI), Washington, DC
Fang J, Liu Y, Luo J, Wang Y, Shewry PR, He G (2009) Allelic variation and genetic diversity of high molecular weight glutenin subunit in Chinese endemic wheats. Euphytica 166:177–182
FAOSTAT (2013) Agriculture organization of the United Nations. Statistical Database, faostat.fao.org
Fehr WR (1987) Principles of cultivar development, vol 2. Macmillan Publishing Co., London
Gaines CS, Finney PF, Fleege LM, Andrews LC (1996) Predicting a hardness measurement using the single-kernel characterization system. Cereal Chem 73:278
Gebre-Mariam H, Tanner DG, Hulluka M (1991) Wheat research in Ethiopia: a historical perspective. Institute of Agricultural Research, Addis Ababa, Ethiopia
Giroux MJ, Sripo T, Gerhardt S, Sherwood JE (2003) Puroindolines: their role in grain hardness and plant defense. Biotechnol Genet Eng Rev 20:277–290
Jackson EA, Morel MH, Sontag-Strohm T, Branlard G, Metakovsky EV, Redaelli R (1996) Proposal for combining the classification systems of alleles of Gli-1 and Glu-3 loci in bread wheat. J Genet Breed 50:321–336
Jin H, Yan J, Peña RJ, Xia XC, Morgounov A, Han LM, Zhang Y, He ZH (2011) Molecular detection of high-and low-molecular-weight glutenin subunit genes in common wheat cultivars from 20 countries using allele-specific markers. Crop Pasture Sci 62:746–754
Jolly CJ, Rahman S, Kortt AA, Higgins TJV (1993) Characterization of the wheat mr 15000 “grain-softness protein” and analysis of the relationship between its accumulation in the whole seed and grain softness. Theor Appl Genet 86:589–597
Kent NL, Evers AD (1994) Kent’s technology of cereals. An introduction for students of food science and agriculture, 4th edn. Pergamon Publications, Oxford
Liu Y, Xiong ZY, He YG, Shewry PR, He G (2007) Genetic diversity of HMW glutenin subunit in Chinese common wheat landraces from Hubei province. Genetic Res Crop Evol 54:865–874
McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Appels R, Xia XC (2013) Catalog of gene symbols for wheat. MacGene. www.shigen.nig.ac.jp/wheat/komugi/genes
Morris CF (2002) Puroindolines: the molecular genetic basis of wheat grain hardness. Plant Mol Biol 48:633–647
Morris CF, Greenblatt GA, Bettge AD, Malkawi HI (1994) Isolation and characterization of multiple forms of friabilin. J Cereal Sci 20:167–174
Nakamura H (2000) Allelic variation at high-molecular-weight glutenin subunit loci, glu-A1, glu-B1 and glu-D1, in Japanese and Chinese hexaploid wheats. Euphytica 112:187–193
Nevo E, Payne PI (1987) Wheat storage proteins: diversity of HMW glutenin subunits in wild emmer from Israel. Theor Appl Genet 74:827–836
Njau PN, Wanyera R, Macharia GK, Macharia J, Singh R, Keller B (2009) Resistance in Kenyan bread wheat to recent Eastern African isolate of stem rust Ug99. J Plant Breed Crop Sci 1:22–27
Osborne TB (1907) The proteins of the wheat kernel. Carnegie Institution of Washington, Washington, DC
Pasha I, Anjum FM, Morris CF (2010) Grain hardness: a major determinant of wheat quality. Food Sci Technol Int 16:511–522
Payne PI (1987) Genetics of wheat storage protein and the effect of allelic variation on pan bread quality. Annu Rev Plant Physiol 38:141–153
Payne PI, Corfield K (1979) Subunit composition of wheat glutenin proteins isolated by gel filtration in a dissociating medium. Planta 145:83–88
Payne PI, Lawrence GJ (1983) Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, and Glu-D1 which code for high molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11:29–35
Payne PI, Jackson EA, Holt LM, Law CN (1984) Genetic linkage between endosperm storage protein genes on each of the short arms of chromosomes 1A and 1B in wheat. Theor Appl Genet 67:235–243
Peña RJ, Curtis BC, Rajaram S (2002) Wheat for bread and other foods. Bread wheat improvement and production. FAO, Rome, pp 483–542
Peña RJ, Gonzalez-Santoyo H, Cervantes F (2004) Relationship between Glu-D1/Glu-B3 allelic combinations and bread-making quality-related parameters commonly used in wheat breeding. In: Masci S, Lafiandra D (eds) Proceedings of 8th Gluten Workshop, Viterbo, Italy, pp 156–157
Rahman S, Christopher J, John H, Wallosheck A (1994) Cloning of a wheat 15-kDa grain softness protein. Eur J Biochem 223:917–925
Shahnejat-Bushehri A, Masoud G, Bahman Y (2006) The high molecular weight glutenin subunit composition in old and modern bread wheats cultivated in Iran. Crop Pasture Sci 57:1109–1114
Shewry PR, Halford NG, Tatham AS (1989) The high molecular weight subunits of wheat, barley and rye: genetics, molecular biology, chemistry and role in wheat gluten structure and functionality. In: Oxford surveys of plant molecular and cell biology, vol. 6. Oxford University Press, Oxford, pp 163–219
Shewry PR, Halford NG, Tatham AS (1992) High molecular weight subunits of wheat glutenin. J Cereal Sci 15:105–120
Singh NK, Shepherd KW, Cornish GB (1991) A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. J Cereal Sci 14:203–208
Singh RP, Hodson DP, Huerta-Espino J, Jin Y, Njau P, Wanyera R, Herrera-Foessel SA, Ward RW (2008) Will stem rust destroy the world’s wheat crop? Adv Agron 98:271–309
Slade AJ, Susan IF, Dayna L, Michael NS, Daniel F (2005) A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING. Nat Biotechnol 23:75–81
Tarekegne A, Labuschagne MT (2005) Relationship between high molecular weight glutenin subunit composition and gluten quality in Ethiopian-grown bread and durum wheat cultivars and lines. J Agron Crop Sci 191:300–307
Tester M, Langridge P (2010) Breeding technologies to increase crop production in a changing world. Science 327:818–822
Tohver M (2007) High molecular weight glutenin subunit composition of some Nordic and middle European wheats. Genetic Res Crop Evol 54:67–81
Veraverbeke WS, Delcour JA (2002) Wheat protein composition and properties of wheat glutenin in relation to bread making functionality. Crit Rev Food Sci Nutr 42:179–208
Weising KH, Wolf NK, Günter K (2005) DNA fingerprinting in plants: principles, methods, and applications. CRC Press, Boca Raton
Acknowledgments
We thank Dr. Craig Morris for providing the puroindoline standards. The help provided by Gloria Nygard in PAGE analysis was gratefully appreciated.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Macharia, G.K., Peña, R.J., Simsek, S. et al. Variation at glutenin subunit loci, single kernel characterization and evaluation of grain protein in East African bread wheat varieties. Euphytica 197, 409–421 (2014). https://doi.org/10.1007/s10681-014-1077-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10681-014-1077-5