Abstract
This study was to determine the agronomic and genetic characteristics of a novel rice dominant dwarf mutant 986083D (japonica) and its potential in breeding. 986083D derived from the anther culture of an autotetraploid indica/japonica hybrid and its progeny segregated into normal and dwarf plants. Homozygous and heterozygous 986083D plants looked similar phenotypically, showing shortened stature, erect leaves, more tillers and poor fertility. The segregation ratio of dwarf to normal plants fit the expected 3:1 by χ2-test in 77 out of 88 tested lines. Crosses between homozygous 986083D and eight other rice varieties had uniform semi-dwarf F1 plants. The F1 plants from crosses between heterozygous 986083D and five other varieties had normal and semi-dwarf plants close to the expected ratio of 1:1. The reduction of plant height in F1 plants ranged from 40.0 to 53.5% in a subtropical environment and from 37.5 to 48.2% in a temperate environment. 986083D showed moderate sensitivity to exogenously applied GA3 in terms of elongation of shoots and induction of α-amylase activity in the endosperm. Linkage analysis showed that the dominant dwarf gene (designated as Dx) in 986083D was not allelic to D53. Dx was roughly mapped to the short arm of chromosome 8. All results showed that 986083D was a novel mutant controlled by single dominant gene, providing a valuable material in rice breeding.
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Acknowledgements
This study was supported by China National Biotechnology Research Program (grant 96c01-01-06), the Key Biotechnology Research Program of the Ministry of Agriculture of China (Bio-02-01-05), China National High-Tech Research and Development ‘863’ Programs (grant BH01-02-01-03 and 2006AA100101) and ‘973’ Program (grant 2006CB100201). We thank Ms. Nancy Blake for her comments on our manuscript.
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Ruizhen Qin, Yang Qiu contributed equally to this work.
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Qin, R., Qiu, Y., Cheng, Z. et al. Genetic analysis of a novel dominant rice dwarf mutant 986083D. Euphytica 160, 379–387 (2008). https://doi.org/10.1007/s10681-007-9548-6
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DOI: https://doi.org/10.1007/s10681-007-9548-6