Environmental Monitoring and Assessment

, Volume 186, Issue 2, pp 1253–1260

Antibiotic-resistant E. coli in surface water and groundwater in dairy operations in Northern California

  • Xunde Li
  • Naoko Watanabe
  • Chengling Xiao
  • Thomas Harter
  • Brenda McCowan
  • Yingjia Liu
  • Edward R. Atwill
Article

DOI: 10.1007/s10661-013-3454-2

Cite this article as:
Li, X., Watanabe, N., Xiao, C. et al. Environ Monit Assess (2014) 186: 1253. doi:10.1007/s10661-013-3454-2

Abstract

Generic Escherichia coli was isolated from surface water and groundwater samples from two dairies in Northern California and tested for susceptibility to antibiotics. Surface samples were collected from flush water, lagoon water, and manure solids, and groundwater samples were collected from monitoring wells. Although E. coli was ubiquitous in surface samples with concentrations ranging from several hundred thousand to over a million colony-forming units per 100 mL of surface water or per gram of surface solids, groundwater under the influence of these high surface microbial loadings had substantially fewer bacteria (3- to 7-log10 reduction). Among 80 isolates of E. coli tested, 34 (42.5 %) were resistant to one or more antibiotics and 22 (27.5 %) were multi-antibiotic resistant (resistant to ≥3 antibiotics), with resistance to tetracycline, cefoxitin, amoxicillin/clavulanic acid, and ampicillin being the most common. E. coli isolates from the calf hutch area exhibited the highest levels of multi-antibiotic resistance, much higher than isolates in surface soil solids from heifer and cow pens, flush alleys, manure storage lagoons, and irrigated fields. Among E. coli isolates from four groundwater samples, only one sample exhibited resistance to ceftriaxone, chloramphenicol, and tetracycline, indicating the potential of groundwater contamination with antibiotic-resistant bacteria from dairy operations.

Keywords

DairyWaterE. coliAntibiotic resistance

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Xunde Li
    • 1
  • Naoko Watanabe
    • 2
  • Chengling Xiao
    • 1
  • Thomas Harter
    • 2
  • Brenda McCowan
    • 1
  • Yingjia Liu
    • 1
  • Edward R. Atwill
    • 1
  1. 1.Department of Population Health and Reproduction, School of Veterinary MedicineUniversity of California, DavisDavisUSA
  2. 2.Department of Land, Air, and Water ResourcesUniversity of California, DavisDavisUSA