Abstract
Quantitative PCR revealed that Tomato chlorotic dwarf viroid (TCDVd) was present in substantial amounts in viroid-infected tomato flowers. Healthy tomato plants were arranged in two different glasshouses, and plants were mechanically inoculated with TCDVd. Bumblebees (Bombus ignitus) were then introduced into the glasshouses to reveal whether the viroid was transmitted from infected source plants to neighbouring healthy plants. TCDVd infection was found in neighbouring tomato plants more than 1 month after the introduction of the bees, some of which expressed symptoms, in both glasshouses. Thus, bumblebees transmitted TCDVd from tomato to tomato by pollination activities.
References
Antignus, Y., Lachman, O., Pearlsman, M., Gofman, R., & Bar-Joseph, M. (2002). A new disease of greenhouse tomatoes in Israel caused by a distinct strain of Tomato apical stunt viroid (TASVd). Phytoparasitica, 30, 502–510.
Antignus, Y., Lachman, O., & Pearlsman, M. (2007). Spread of Tomato apical stunt viroid (TASVd) in greenhouse tomato crops is associated with seed transmission and bumble bee activity. Plant Disease, 91, 47–50.
Behjatnia, S. A. A., Dry, I. B., Krake, L. R., Conde, B. D., Connelly, M. I., Randles, J. W., et al. (1996). New Potato spindle tuber viroid and Tomato leaf curl geminivirus strains from a wild Solanum sp. Phytopathology, 86, 880–886.
Diener, T. O. (2001). The viroid: biological oddity or evolutionary fossil? Advances in Virus Research, 57, 137–184.
Galindo, J., Smith, D. R., & Diener, T. O. (1982). Etiology of planta macho, a viroid disease of tomato. Phytopathology, 72, 49–54.
James, T., Mulholland, V., Jeffries, C., & Chard, J. (2008). First report of Tomato chlorotic dwarf viroid infecting commercial petunia stocks in the United Kingdom. Plant Pathology, 57, 400.
Kryczyński, S., Paduch-Cichal, E., & Skrzeczkowski, L. J. (1988). Transmission of three viroids through seed and pollen of tomato plants. Journal of Phytopathology, 121, 51–57.
Matsushita, Y., Kanda, A., Usugi, T., & Tsuda, S. (2008). First report of a Tomato chlorotic dwarf viroid disease on tomato plants in Japan. Journal of General Plant Pathology, 74, 182–184.
Matsushita, Y., Usugi, T., & Tsuda, S. (2009). Host range and properties of Tomato chlorotic dwarf viroid. European Journal of Plant Pathology, 124, 349–352.
Mumford, R. A., Jarvis, B., & Skelton, A. (2004). The first report of Potato spindle tuber viroid (PSTVd) in commercial tomatoes in the UK. Plant Pathology, 53, 242.
Okada, K., Kusakari, S., Kawaratani, M., Neguro, J., Ohki, S. T., & Osaki, T. (2000). Tobacco mosaic virus is transmissible from tomato to tomato by pollinating bumblebees. Journal of General Plant Pathology, 66, 71–74.
Shipp, J. L., Buitenhuis, R., Stobbs, L., Wang, K., Kim, W. S., & Ferguson, G. (2008). Vectoring of Pepino mosaic virus by bumble-bees in tomato greenhouses. Annals of Applied Biology, 153, 149–155.
Singh, R. P., Nie, X., & Singh, M. (1999). Tomato chlorotic dwarf viroid: an evolutionary link in the origin of pospiviroids. Journal of General Virology, 80, 2823–2828.
Singh, R. P. (2006). Reassessment of the presence of viroid species of the genus Pospiviroid in infected floral parts, using reverse transcription-polymerase chain reaction and infectivity assays. Canadian Journal of Plant Pathology, 28, 242–249.
Singh, R. P., & Dilworth, A. D. (2009). Tomato chlorotic dwarf viroid in the ornamental plant Vinca minor and its transmission through tomato seed. European Journal of Plant Pathology, 123, 111–116.
Velthuis, H. H. W., & van Doorn, A. (2006). A century of advances in bumblebee domestication and the economic and environmental aspects of its commercialization for pollination. Apidologie, 37, 421–451.
Verhoeven, J. Th J., Jansen, C. C. C., Willemen, T. M., Kox, L. F. F., Owens, R. A., & Roenhorst, J. W. (2004). Natural infections of tomato by Citrus exocortis viroid, Columnea latent viroid, Potato spindle tuber viroid and Tomato chlorotic dwarf viroid. European Journal of Plant Pathology, 110, 823–831.
Acknowledgements
We are grateful to Dr. Taeko Takeuchi of Chiba Prefectural Agriculture and Forestry Research Centre, Japan for her advice. This work was supported in part by Research Project (No. 1976) for the Utilisation of Advanced Technologies in Agriculture, Forestry and Fisheries, of the Ministry of Agriculture, Forestry and Fisheries of Japan.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Matsuura, S., Matsushita, Y., Kozuka, R. et al. Transmission of Tomato chlorotic dwarf viroid by bumblebees (Bombus ignitus) in tomato plants. Eur J Plant Pathol 126, 111–115 (2010). https://doi.org/10.1007/s10658-009-9515-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10658-009-9515-2