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Hsp70 and Hsp90 expression in citrus and pepper plants in response to Xanthomonas axonopodis pv. citri

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Abstract

Hsp70 and Hsp90 expression in response to high and low temperatures was studied in orange, the host plant of Xanthomonas axonopodis pv. citri and in a non-host resistant plant, pepper. As expected in both plants, the expression of these chaperones was induced at high temperatures while at cold temperatures the response was chaperone and plant-dependent. Expression of Hsp70 and Hsp90 was analysed during citrus canker and no changes in their levels could be observed whereas pepper plants infiltrated with the phytopathogen showed an increase in the levels of both chaperones. These results suggest that no changes in Hsp70 and Hsp90 expression are necessary during the disease while they are increased in non-host resistance.

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Abbreviations

hpi:

hours post-infiltration

HR:

hypersensitive response

Hsp70:

Heat-shock proteins of 70 kDa

Hsp90:

Heat-shock proteins of 90 kDa

Hsps:

Heat-shock proteins

References

  • Brunings, A. M., & Gabriel, D. W. (2003). Xanthomonas citri: breaking the surface. Molecular Plant Pathology, 4, 141–157.

    Article  CAS  Google Scholar 

  • Dunger, G., Arabolaza, L. N., Gottig, N., Orellano, E. G., & Ottado, J. (2005). Participation of Xanthomonas axonopodis pv. citri hrp cluster in citrus canker and in non-host plant responses. Plant Pathology, 54, 781–788.

    Article  CAS  Google Scholar 

  • Dunger, G., Relling, V. M., Tondo, M. L., Barreras, M., Ielpi, L., Orellano, E. G., et al. (2007). Xanthan is not essential for pathogenicity in citrus canker but contributes to Xanthomonas epiphytic survival. Archives of Microbiology, 188, 127–135.

    Article  PubMed  CAS  Google Scholar 

  • Hubert, D. A., Tornero, P., Belkhadir, Y., Krishna, P., Takahashi, A., Shirasu, K., et al. (2003). Cytosolic HSP90 associates with and modulates the Arabidopsis RPM1 disease resistance protein. EMBO Journal, 22, 5679–5689.

    Article  PubMed  CAS  Google Scholar 

  • Kanzaki, H., Saitoh, H., Ito, A., Fujisawa, S., Kamoun, S., Katou, S., et al. (2003). Cytosolic HSP90 and HSP70 are essential components of INF1-mediated hypersensitive response and non-host resistance to Pseudomonas cichorii in Nicotiana benthamiana. Molecular Plant Pathology, 4, 383–391.

    Article  CAS  Google Scholar 

  • Krishna, P., Sacco, M., Cherutti, J. F., & Hill, S. (1995). Cold-induced accumulation of hsp90 transcripts in Brassica napus. Plant Physiology, 107, 915–923.

    PubMed  CAS  Google Scholar 

  • Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685.

    Article  PubMed  CAS  Google Scholar 

  • Li, Q.-B., & Guy, C. L. (1998). The organization and evolution of the spinach stress 70 molecular chaperone gene family. Plant Cell, 10, 539–556.

    PubMed  Google Scholar 

  • Li, Q.-B., Haskell, D. W., & Guy, C. L. (1999). Coordinate and non-coordinate expression of the stress 70 family and other molecular chaperones at high and low temperature in spinach and tomato. Plant Molecular Biology, 39, 21–34.

    Article  PubMed  Google Scholar 

  • Liu, Y., Burch-Smith, T., Schiff, M., Feng, S., & Dinesh-Kumar, S. P. (2004). Molecular chaperone Hsp90 associates with resistance protein N and its signaling proteins SGT1 and Rar1 to modulate an innate immune response in plants. Journal of Biological Chemistry, 279, 2101–2108.

    Article  PubMed  CAS  Google Scholar 

  • Liu, D., Zhang, X., Cheng, Y., Takano, T., & Liu, S. (2006). rHsp90 gene expression in response to several environmental stresses in rice (Oryza sativa L.). Plant Physiology and Biochemistry, 44, 380–386.

    Article  PubMed  CAS  Google Scholar 

  • Lu, R., Malcuit, I., Moffett, P., Ruiz, M. T., Peart, J., Wu, A. J., et al. (2003). High throughput virus-induced gene silencing implicates heat shock protein 90 in plant disease resistance. EMBO Journal, 22, 5690–5699.

    Article  PubMed  CAS  Google Scholar 

  • Marrs, K. A. E., Casey, S., Capitant, S. A., Bouchard, R. A., Dietrich, P. S., Mettler, I. J., et al. (1993). Characterization of two maize hsp90 heat shock protein genes and expression during heat shock, embryogenesis, and pollen development. Developmental Genetics, 14, 27–41.

    Article  PubMed  CAS  Google Scholar 

  • Milioni, D., & Hatzopoulos, P. (1997). Genomic organization of hsp90 gene family in Arabidopsis. Plant Molecular Biology, 35, 955–961.

    Article  PubMed  CAS  Google Scholar 

  • Ortiz, C., & Cardemil, L. (2001). Heat-shock responses in two leguminous plants: a comparative study. Journal of Experimental Botany, 52, 1711–1719.

    Article  PubMed  CAS  Google Scholar 

  • Queitsch, C., Sangster, T. A., & Lindquist, S. (2002). Hsp90 as a capacitor of phenotypic variation. Nature, 417, 618–624.

    Article  PubMed  CAS  Google Scholar 

  • Rutherford, S. L. (2003). Between genotype and phenotype: protein chaperones and evolvability. Nature Reviews in Genetics, 4, 263–274.

    Article  CAS  Google Scholar 

  • Rutherford, S. L., & Lindquist, S. (1998). Hsp90 as a capacitor for morphological evolution. Nature, 396, 336–342.

    Article  PubMed  CAS  Google Scholar 

  • Salathia, N., & Queitsch, C. (2007). Molecular mechanisms of canalization: Hsp90 and beyond. Journal of Bioscience, 32, 457–463.

    Article  CAS  Google Scholar 

  • Sangster, T. A., & Queitsch, C. (2005). The HSP90 chaperone complex, an emerging force in plant development and phenotypic plasticity. Current Opinion in Plant Biology, 8, 86–92.

    Article  PubMed  CAS  Google Scholar 

  • Sedmak, J. J., & Grossberg, S. E. (1977). A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250. Analytical Biochemistry, 79, 544–552.

    Article  PubMed  CAS  Google Scholar 

  • Sung, D. Y., & Guy, C. L. (2003). Physiological and molecular assessment of altered expression of Hsc70-1 in Arabidopsis. Evidence for pleiotropic consequences. Plant Physiology, 132, 979–987.

    Article  PubMed  CAS  Google Scholar 

  • Sung, D. Y., Vierling, E., & Guy, C. L. (2001). Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family. Plant Physiology, 126, 789–800.

    Article  PubMed  CAS  Google Scholar 

  • Swindell, W. R., Huebner, M., & Weber, A. P. (2007). Transcriptional profiling of Arabidopsis heat shock proteins and transcription factors reveals extensive overlap between heat and non-heat stress response pathways. BMC Genomics, 8, 125–139.

    Article  PubMed  Google Scholar 

  • Takahashi, A., Casais, C., Ichimura, K., & Shirasu, K. (2003). HSP90 interacts with RAR1 and SGT1 and is essential for RPS2-mediated disease resistance in Arabidopsis. Proceedings of the National Academy of Sciences, 100, 11777–11782.

    Article  CAS  Google Scholar 

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Acknowledgements

The authors wish to thank Catalina Anderson, Gastón Alanis and Rubén Díaz Vélez for the citrus plants used in this work and Natalia Gottig for critical reading of the manuscript. EGO and JO are staff members and BSG is a Fellow of the Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina). This work was funded by Agencia Nacional de Promoción Científica y Tecnológica (PICT 01-12783 to EGO and PICT 01-14651 to JO).

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Authors and Affiliations

  1. Molecular Biology Division, IBR (Instituto de Biología Molecular y Celular de Rosario), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK, Rosario, Argentina

    Betiana S. Garavaglia, Cecilia G. Garofalo, Elena G. Orellano & Jorgelina Ottado

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  1. Betiana S. Garavaglia
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  2. Cecilia G. Garofalo
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  3. Elena G. Orellano
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  4. Jorgelina Ottado
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Correspondence to Jorgelina Ottado.

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Garavaglia, B.S., Garofalo, C.G., Orellano, E.G. et al. Hsp70 and Hsp90 expression in citrus and pepper plants in response to Xanthomonas axonopodis pv. citri . Eur J Plant Pathol 123, 91–97 (2009). https://doi.org/10.1007/s10658-008-9336-8

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  • DOI: https://doi.org/10.1007/s10658-008-9336-8

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