Abstract
Microbial communities have largely existed in the seagrass meadows. A total of 496 strains of the bacteria in the seagrass meadows, which belonged to 50 genera, were obtained by the plate cultivation method from three sites of Xincun Bay, South China Sea. The results showed that Bacillales and Vibrionales accounted for the highest proportions of organisms in all communities. The diversity of the bacteria in the sediment was higher than that associated with seagrass. Thalassia hemperichii possessed the highest abundance of bacteria, followed by Enhalus acoroides and Cymodocea rotundata. Robust seasonal dynamics in microbial community composition were also observed. It was found that microbial activities were closely tied to the growth stage of the seagrass. The microbial distribution was the lowest in site 3. The abundance of the bacteria was linked to the interactions between bacteria and plants, the condition of plant and even the coastal water quality and the nutrition level in the sediment.
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References
An QL (2014) Complete genome sequence of Kosakonia sacchari type strain SP1T. Stand Genomic Sci 9:1311–1318
Andrews JH, Harris RF (2000) The ecology and biogeography of microorganisms on plant surfaces. Annu Rev Phytopathol 38:145–180
Aravindraja C, Viszwapriya D, Pandian SK (2013) Ultradeep 16S rRNA sequencing analysis of geographically similar but diverse unexplored marine samples reveal varied bacterial community composition. PLoS One 8:e76724
Bolton JH, Fredrickson J, Elliott L, Metting F Jr (1992) Microbial ecology of the rhizosphere. Soil Microb Ecol 27:27–63
Borum J, Sand JK, Binzer T, Pedersen O, Greve TM (2006) Oxygen movement in seagrasses. In: Seagrasses: biology, ecology and conservation. Springer, Netherlands
Brencic A, Winans SC (2005) Detection of and response to signals involved in host-microbe interactions by plant-associated bacteria. Microbiol Mol Biol Rev 69:155–194
Cebrian J, Duarte CM (2001) Detrital stocks and dynamics of the seagrass Posidonia oceanica (L.) Delile in the Spanish Mediterranean. Aquat Bot 70:295–309
Christiaen B, Mcdonald A, Cebrian J, Ortmann AC (2013) Response of the microbial community to environmental change during seagrass transplantation. Aquat Bot 109:31–38
Dietz H, Fischer M, Schmid B (1999) Demographic and genetic invasion history of a 9-year-old roadside population of Bunias orientalis L. (Brassicaceae). Oecologia 120:225–234
Escobar-Nino A, Luna C, Luna D, Marcos AT, Canovas D, Mellado E (2014) Selection and characterization of biofuel-producing environmental bacteria isolated from vegetable oil-rich wastes. PLoS One 9:e104063
Gallego S, Vila J, Nieto JM, Urdiain M, Rossello-Mora R, Grifoll M (2010) Breoghania corrubedonensis gen. nov. sp. nov., a novel alphaproteobacterium isolated from a Galician beach (NW Spain) after the Prestige fuel oil spill, and emended description of the family Cohaesibacteraceae and the species Cohaesibacter gelatinilyticus. Syst Appl Microbiol 33:316–321
Grime JP et al (2000) The response of two contrasting limestone grasslands to simulated climate change. Science 289:762–765
Hamisi MI, Lyimo TJ, Muruke M (2007) Cyanobacterial occurrence and diversity in seagrass meadows in coastal Tanzania. West Indian Ocean J Mar Sci 3:113–122
Hamisi MI, Lyimo TJ, Muruke MH, Bergman B (2009) Nitrogen fixation by epiphytic and epibenthic diazotrophs associated with seagrass meadows along the Tanzanian coast, Western Indian Ocean. Aquat Microb Ecol 57:33–42
Hirano SS, Upper CD (2000) Bacteria in the leaf ecosystem with emphasis on pseudomonas syringae—a pathogen, ice nucleus, and epiphyte. Microbiol Mol Biol Rev 64:624–653
Holmer M, Bondgaard EJ (2001) Photosynthetic and growth response of eelgrass to low oxygen and high sulfide concentrations during hypoxic events. Aquat Bot 70:29–38
Jensen SI, Kuhl M, Glud RN, Jørgensen LB, Prieme A (2005) Oxic microzones and radial oxygen loss from roots of Zostera marina. Mar Ecol Prog Ser 293:49–58
Jose PA (2014) Molecular phylogeny and plant growth promoting traits of endophytic bacteria isolated from roots of seagrass Cymodocea serrulata. Indian J Med Sci 43:571–579
Kim OS et al (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721
Kumar PS, Khan AB (2013) The distribution and diversity of benthic macroinvertebrate fauna in Pondicherry mangroves, India. Aquat Biosyst 9:15
Kurtz JC, Yates DF, Macauley JM, Quarles RL, Genthner FJ, Chancy CA, Devereux R (2003) Effects of light reduction on growth of the submerged macrophyte Vallisneria americana and the community of root-associated heterotrophic bacteria. J Exp Mar Biol Ecol 291:199–218
Lavery PS, Mateo MA, Serrano O, Rozaimi M (2013) Variability in the carbon storage of seagrass habitats and its implications for global estimates of blue carbon ecosystem service. PLoS One 8:e73748
Leon FD et al (2011) Phenotypic characteristics of isolates of aspergillus section Fumigati from different geographic origins and their relationships with genotypic characteristics. BMC Infect Dis 11:116
Loreau M et al (2001) Biodiversity and ecosystem functioning: current knowledge and future challenges. Science 294:804–808
Milbrandt EC, Greenawalt-Boswell J, Sokoloff PD (2008) Short-term indicators of seagrass transplant stress in response to sediment bacterial community disruption. Bot Mar 51:103–111
Moriarty D, Pollard P (1982) Diel variation of bacterial productivity in seagrass (Zostera capricorni) beds measured by rate of thymidine incorporation into DNA. Mar Biol 72:165–173
Moulin L, Munive A, Dreyfus B, Boivin-Masson C (2001) Nodulation of legumes by members of the β-subclass of Proteobacteria. Nature 411:948–950
Nielsen JT, Liesack W, Finster K (1999) Desulfovibrio zosterae sp. nov., a new sulfate reducer isolated from surface-sterilized roots of the seagrass Zostera marina. Int J Syst Bacteriol 49:859–865
Nubel U et al (1996) Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. J Bacteriol 178:5636–5643
Pandey S, Sree A, Dash SS, Sethi DP, Chowdhury L (2013) Diversity of marine bacteria producing beta-glucosidase inhibitors. Microb Cell Fact. doi:10.1186/1475-2859-12-35
Smith AC, Kostka JE, Devereux R, Yates DF (2004) Seasonal composition and activity of sulfate-reducing prokaryotic communities in seagrass bed sediments. Aquat Microb Ecol 37:183–195
Sondi I, Salopek-Sondi B (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci 275:177–182
Sundqvist MK, Sanders NJ, Wardle DA (2013) Community and ecosystem responses to elevational gradients: processes, mechanisms, and insights for global change. Annu Rev Ecol Evol Syst 44:261–280
Tolli JD, Sievert SM, Taylor CD (2006) Unexpected diversity of bacteria capable of carbon monoxide oxidation in a coastal marine environment, and contribution of the Roseobacter-associated clade to total CO oxidation. Appl Environ Microbiol 72:1966–1973
Uku J, Bjork M, Bergman B, Diez B (2007) Characterization and comparison of prokaryotic epiphytes associated with three east african seagrasses. J Phycol 43:768–779
Wang DR et al (2012) Distribution of sea-grass resources and existing threat in Hainan Island. Mar Environ Sci 1:008
Weidner S, Arnold W, Stackebrandt E, Pühler A (2000) Phylogenetic analysis of bacterial communities associated with leaves of the seagrass Halophila stipulacea by a culture-independent small-subunit rRNA gene approach. Microb Ecol 39:22–31
Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16s ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
Welsh DT (2000) Nitrogen fixation in seagrass meadows: regulation, plant–bacteria interactions and significance to primary productivity. Ecol Lett 3:58–71
Yang D, Huang DJ (2011) Impacts of Typhoons Tianying and Dawei on seagrass distribution in Xincun Bay, Hainan Province, China. Acta Oceanol Sin 30:32–39
Yang D, Yang C (2009) Detection of seagrass distribution changes from 1991 to 2006 in Xincun Bay, Hainan, with satellite remote sensing. Sensors 9:830–844
Yu ZQ, Deng H, Wu KW, Du J, Ma M (2012) Nutrient contents of dominant seagrass species and their affecting factors in Hainan Province. J East China Normal Univ (Nat Sci) 4:131–141
Acknowledgments
This research was supported by the National Natural Science Foundation of China (No.41276113, No.41276114, No.41006069, No.41406191 and No. 41430966), the National High Technology Research and Development Program of China (No.2012AA092104, No.2013AA092901 and No.2013AA092902), the Strategic Priority Research Program of the Chinese Academy of Sciences (No.XDA11020202), the Science and technology cooperation projects of Sanya (No.2013YD74), and the Sanya Station Database and the Information System of CERN, the Knowledge Innovation Program of the Chinese Academy of Sciences (No.SQ201218), the Open Fund of Key Laboratory for Ecological Environment in Coastal Areas, State Oceanic Administration (No.201304).
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Jiang, YF., Ling, J., Wang, YS. et al. Cultivation-dependent analysis of the microbial diversity associated with the seagrass meadows in Xincun Bay, South China Sea. Ecotoxicology 24, 1540–1547 (2015). https://doi.org/10.1007/s10646-015-1519-4
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DOI: https://doi.org/10.1007/s10646-015-1519-4