Four peptides of molecular weights 1.148, 4.68, 5.41, and 9.086 kDa with antioxidant activity were isolated from chickpea sprout using two-step ion-exchange chromatographic and HPLC techniques for the first time. The partial N-terminal amino acid sequences of the peptide named YZDBCM-1 (9.086 kDa) was determined as H2N1-15-H2N-Ala-Ile-Thr-Cys-Gly-Arg-Val-Ser-Ala-Ala-Leu-Ala-Pro-Pro-Leu using the Edman automated sequencing apparatus, which was a new peptide rich in alanine. It was shown that the antioxidant activity of the peptide YZDBCM-1 was IC50 156.2 μg/mL (17.2 μmol/L) to the free radical of ABTS.
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References
G. R. De Nicola, E. Pagnotta, M. Bagatta, P. Rollin, and R. Iori, J. Biotechnol., 150 S, S1–S576 (2010).
Yu. I. Oshchepkova, E. A. Rogozhin, O. N. Veshkurova, Ts. A. Egorov, Sh. I. Salikhov, and E. V. Grishin, Chem. Nat. Comp., 45, 5 (2009).
N. Ito, S. Fukushima, A. Hagiwara, M. Shibata, and T. Ogiso, J. Natl. Cancer Inst., 70, 343 (1983).
G.-H. Bu, H.-J. Li, G.-Y. Yang, and G.-H. Wang, J. Prog. Vet. Med., 26 (3), 26 (2005).
Q. Y. Lv, Y. Yang, Y. X. Zhao, D. Y. Gu, D. J. He, A. Yili, Q. L. Ma, Zh. Cheng, Y. H. Gao, H. A. Aisa, and Y. Ito, J. Liq. Chromatogr. Relat. Technol., 1, 32, 2879 (2009).
U. K. Laemmli, Nature (London), 227, 680 (1970).
B. P. A. Cammue, K. Thevissen, M. Hendriks, K. Eggermont, I. J. Goderis, P. Proost, J. Van Damme, R. W. Osborn, F. Guerbette, J. C. Kader, and W. F. Broekaert, Plant Physiol., 109, 445 (1995).
P. Coutos-Thevenot, T. Jounenne, O. Maes, F. Guerbette, M. Grosbois, J. P. Le Caer, M. Boulay, A. Deloire, J. C. Kader, and J. Guern, Eur. J. Biochem., 217, 885 (1993).
A. Molina, A. Segura, and F. Garcia-Olmedo, FEBS Lett., 16, 119 (1993).
P. Edman and A. Henschen, Protein Sequence Determination, S. B. Needleman, 2nd Ed., Springer, Berlin, Heidelberg, New York, 1975, p. 232.
A. Yili, H. A. Aisa, X. Imamu, V. V. Maksimov, Zh. F. Ziyavitdinov, O. N. Veshkurova, N. Zh. Sagdiev, and Sh. I. Salikhov, Khim. Prir. Soedin., 588 (2006).
M. M. Bradford, Anal. Biochem., 72, 248 (1976).
Sh. Y. Wang, J. H. Wu, T. B. Ng, X. Y. Ye, and P. F. Rao, Peptides, 25, 1235 (2004).
Acknowledgment
This work was supported by China National Funds for Distinguished Young Scientists (No. 30925045), the Key Project of Knowledge Innovation Program of Chinese Academy of Sciences (No.KGCX2-YW-503), and the CAS/SAFEA International Partnership Program for Creative Research Teams (2008-18).
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Published in Khimiya Prirodnykh Soedinenii, No. 4, July–August, 2012, pp. 578–580.
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Yili, A., Ma, Q.L., Lv, Q.Y. et al. Antioxidant peptides from Cicer arietinum of Xinjiang, China. Chem Nat Compd 48, 643–645 (2012). https://doi.org/10.1007/s10600-012-0332-z
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DOI: https://doi.org/10.1007/s10600-012-0332-z