Abstract
We report the isolation and characterization of 16 microsatellite loci to study the population genetics of the giant kelp, Macrocystis pyrifera. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Of the 37 primer pairs defined, 16 amplified clean polymorphic microsatellites and are described. These loci identified a number of alleles ranging from three to forty (mean = 16.5, and gene diversity ranging from 0.469 to 0.930 (mean = 0.774). The isolation and characterization of these highly polymorphic markers will greatly benefit much needed studies on the molecular ecology of this important macroalga.
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Acknowledgments
We thank E. Hoaglund and T. Crombie for technical assistance. Financial support for this work was provided by the US National Science Foundation grant numbers OCE96-14091, OCE99-82105, and OCE06-20276 and by Fundação para a Ciência e Tecnologia pos-doctoral grant [SFRH/BPD/14945/2004 to F.A.], and grant MEGIKELP [PTDC/MAR/65461/2006].
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Alberto, F., Whitmer, A., Coelho, N.C. et al. Microsatellite markers for the giant kelp Macrocystis pyrifera . Conserv Genet 10, 1915–1917 (2009). https://doi.org/10.1007/s10592-009-9853-9
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DOI: https://doi.org/10.1007/s10592-009-9853-9