Abstract
In the present study, 10 polymorphic microsatellite DNA loci from Atlantic halibut (Hippoglossus hippoglossus) were isolated and characterized. The number of alleles for these loci ranged from 2 to 4 in tested 24 individuals. Observed and expected heterozygosities per locus varied from 0.21 to 0.70 and from 0.31 to 0.65, respectively. Most of these 10 microsatellite loci were successfully amplified and showed polymorphic in five related species. These loci will be useful for the assessment of genetic diversity and population structure of Atlantic halibut.
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Acknowledgements
We are grateful to Dr. Hongyu Ma for his assistance with this work, and this study was supported by State 863 High-technology R&D project of China (2006AA10A403), Taishan Scholar Project of Shandong Province, and scientific foundation of YSFRI, CAFS (2007-qn-13).
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H. Ding and C. Shao contributed equally to this work.
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Ding, H., Shao, C., Liao, X. et al. Ten polymorphic microsatellite loci for the Atlantic halibut (Hippoglossus hippoglossus) and cross-species application in related species. Conserv Genet 10, 611–614 (2009). https://doi.org/10.1007/s10592-008-9589-y
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DOI: https://doi.org/10.1007/s10592-008-9589-y