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Optimized acoustic biochip integrated with microfluidics for biomarkers detection in molecular diagnostics

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Abstract

The development of integrated platforms incorporating an acoustic device as the detection element requires addressing simultaneously several challenges of technological and scientific nature. The present work was focused on the design of a microfluidic module, which, combined with a dual or array type Love wave acoustic chip could be applied to biomedical applications and molecular diagnostics. Based on a systematic study we optimized the mechanics of the flow cell attachment and the sealing material so that fluidic interfacing/encapsulation would impose minimal losses to the acoustic wave. We have also investigated combinations of operating frequencies with waveguide materials and thicknesses for maximum sensitivity during the detection of protein and DNA biomarkers. Within our investigations neutravidin was used as a model protein biomarker and unpurified PCR amplified Salmonella DNA as the model genetic target. Our results clearly indicate the need for experimental verification of the optimum engineering and analytical parameters, in order to develop commercially viable systems for integrated analysis. The good reproducibility of the signal together with the ability of the array biochip to detect multiple samples hold promise for the future use of the integrated system in a Lab-on-a-Chip platform for application to molecular diagnostics.

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References

  • M.U. Ahmed, I. Saaem, P.C. Wu, A.S. Brown, Crit. Rev. Biotechnol. 34, 180–196 (2014)

    Article  Google Scholar 

  • D. Ballantine, Jr., S.J. Martin, A.J. Ricco, G.C. Frye, H. Wohltjen, R.M. White and E.T. Zellers, Acoustic wave sensors: Theory, design and physico-chemical applications (Academic Press, San Diego, 1996), p. 436

  • P. Bröker, K. Lücke, M. Perpeet, T.M.A. Gronewold, Sensors Actuators B Chem. 165, 1–6 (2012)

    Article  Google Scholar 

  • C.D. Chin, V. Linder, S.K. Sia, Lab Chip 12, 2118–2134 (2012)

    Article  Google Scholar 

  • J. Du, G.L. Harding, J.A. Ogilvy, P.R. Dencher, M. Lake, Sens. Actuators, A 56, 211–219 (1996)

    Article  Google Scholar 

  • E. Gizeli, Smart Mater. Struct. 6, 700–706 (1997)

    Article  Google Scholar 

  • E. Gizeli, N.J. Goddard, C.R. Lowe, A.C. Stevenson, Sensors Actuators B Chem. 6, 131–137 (1992)

    Article  Google Scholar 

  • T.M. Gronewold, Anal. Chim. Acta 603(2), 119–128 (2007)

    Article  Google Scholar 

  • T.M. Gronewold, A. Baumgartner, E. Quandt, M. Famulok, Anal. Chem. 78(14), 4865–4871 (2006)

    Article  Google Scholar 

  • I. Hein, G. Flenka, M. Krassnig, M. Wagner, J. Microbiol. Methods 66, 538–547 (2006)

    Article  Google Scholar 

  • F. Josse, F. Bender, R.W. Carmose, Anal. Chem. 73(24), 5937–5944 (2001)

    Article  Google Scholar 

  • Y.W. Kim, M.T. Meyer, A. Berkovich, S. Subramanian, A.A. Iliadis, W.E. Bentley, R. Ghodssi, Sens. Actuators, A 238, 140–149 (2016)

    Article  Google Scholar 

  • G. Kovacs, M.J. Vellekoop, R. Haueis, G.W. Lubking, A. Venema, Sens. Actuators, A 43, 38–43 (1994)

    Article  Google Scholar 

  • S. Krishnamoorthy, A.A. Iliadis, T. Bei, G.P. Chrousos, Biosens. Bioelectron. 24(2), 313–318 (2008)

    Article  Google Scholar 

  • K. Länge, M. Rapp, Sensors Actuators B Chem. 142, 39–43 (2009)

    Article  Google Scholar 

  • K. Länge, G. Blaess, A. Voigt, R. Götzen, M. Rapp, Biosens. Bioelectron. 22(2), 227–232 (2006)

    Article  Google Scholar 

  • K. Länge, B.E. Rapp, M. Rapp, Anal. Bioanal. Chem. 391(5), 1509–1519 (2008)

    Article  Google Scholar 

  • J. Lee, Y.S. Choi, Y. Lee, H.J. Lee, J.N. Lee, et al., Anal. Chem. 83(22), 8629–8635 (2011)

    Article  Google Scholar 

  • W. Lee, J. Jung, Y.K. Hahn, S.K. Kim, Y. Lee, et al., Analyst 138(9), 2558–2566 (2013)

    Article  Google Scholar 

  • D. Matatagui, D. Moynet, M.J. Fernandez, J. Fontecha, J.P. Esquivel, et al., Sensors Actuators B Chem. 185, 218–224 (2013)

    Article  Google Scholar 

  • D. Matatagui, J.L. Fontecha, M.J. Fernandez, I. Gracia, C. Cane, J.P. Santos, M.C. Horrillo, Sensors 14, 12658–12669 (2014)

    Article  Google Scholar 

  • G. McHale, F. Martin, M.I. Newton, J. Appl. Phys. 92, 3368–3379 (2002)

    Article  Google Scholar 

  • K. Mitsakakis, E. Gizeli, Anal. Chim. Acta 699(1), 1–5 (2011a)

    Article  Google Scholar 

  • K. Mitsakakis, E. Gizeli, Biosens. Bioelectron. 26(11), 4579–4584 (2011b)

    Article  Google Scholar 

  • K. Mitsakakis, A. Tserepi, E. Gizeli, J. Microelectromech. Syst. 17, 1010–1019 (2008)

    Article  Google Scholar 

  • K. Mitsakakis, A. Tsortos, E. Gizeli, Analyst 139(16), 3918–3925 (2014)

    Article  Google Scholar 

  • G. Papadakis, E. Gizeli, Anal. Methods 6, 363–371 (2014)

    Article  Google Scholar 

  • G. Papadakis, A. Tsortos, E. Gizeli, Biosens. Bioelectron. 25(4), 702–707 (2009)

    Article  Google Scholar 

  • G. Papadakis, A. Tsortos, F. Bender, E.E. Ferapontova, E. Gizeli, Anal. Chem. 84, 1854–1861 (2012)

    Article  Google Scholar 

  • G. Papadakis, A. Tsortos, A. Kordas, I. Tiniakou, E. Morou, J. Vontas, D. Kardassis, E. Gizeli, Sci Rep 3, 2033 (2013)

    Article  Google Scholar 

  • G. Papadakis, N. Skandalis, A. Dimopoulou, P. Glynos, E. Gizeli, PLoS One 10, e0132773 (2015)

    Article  Google Scholar 

  • M. Perpeet, S. Glass, T. Gronewold, A. Kiwitz, A. Malavé, et al., Anal. Lett. 39, 1747–1757 (2006)

    Article  Google Scholar 

  • A. Rasmusson, E. Gizeli, J. Appl. Phys. 90, 5911–5914 (2001)

    Article  Google Scholar 

  • K. Saha, F. Bender, E. Gizeli, Anal. Chem. 75(4), 835–842 (2003)

    Article  Google Scholar 

  • O. Tamarin, S. Comeau, C. Déjous, D. Moynet, D. Rebière, J. Bezian, J. Pistré, Biosens. Bioelectron. 18(5–6), 755–763 (2003)

    Article  Google Scholar 

  • A. Tsortos, G. Papadakis, K. Mitsakakis, K. Melzak, E. Gizeli, Biophys. J. 94(7), 2706–2715 (2008)

    Article  Google Scholar 

  • A. Tsortos, A. Grammoustianou, R. Lymbouridou, G. Papadakis, E. Gizeli, Chem. Commun. 51, 11504–11507 (2015)

    Article  Google Scholar 

  • A. Tsortos, G. Papadakis, E. Gizeli, Anal. Chem. 88, 6472–6478 (2016)

    Article  Google Scholar 

  • I. Voiculescu, A.N. Nordin, Biosens Bioelectron 33, 1–9 (2012)

    Article  Google Scholar 

  • J. Wu, Z. He, Q. Chen, J.-M. Lin, Trac-Trend Anal Chem 80, 213–231 (2016)

    Article  Google Scholar 

Download references

Acknowledgements

The authors would like to acknowledge the financial support of the European Commission through FP7-ICT-2011.3.2 (LOVE-FOOD, No 317742) and HORIZON2020-ICT 28-2015 (LoveFood2Market, No 687681) grants.

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Authors and Affiliations

  1. Institute of Molecular Biology and Biotechnology-FORTH, 100 N. Plastira Str, 70013, Heraklion, Greece

    G. Papadakis & E. Gizeli

  2. FEMTO-ST Time & Frequency/SENSeOR, 26 rue de l’Epitaphe, 25000, Besançon, France

    J. M. Friedt

  3. SENSeOR SAS, Temis Innovation, 18 rue Alain Savary, 25000, Besançon, France

    J. M. Friedt & D. Rabus

  4. Jobst Technologies GmbH, Engesserstraße 4b, 79108, Freiburg, Germany

    M. Eck & G. Jobst

  5. Department of Biology, University of Crete, 71409, Vassilika Vouton, Greece

    E. Gizeli

Authors
  1. G. Papadakis
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  2. J. M. Friedt
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  3. M. Eck
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  4. D. Rabus
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  5. G. Jobst
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  6. E. Gizeli
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Correspondence to G. Papadakis or E. Gizeli.

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Papadakis, G., Friedt, J.M., Eck, M. et al. Optimized acoustic biochip integrated with microfluidics for biomarkers detection in molecular diagnostics. Biomed Microdevices 19, 16 (2017). https://doi.org/10.1007/s10544-017-0159-2

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  • DOI: https://doi.org/10.1007/s10544-017-0159-2

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