Biomedical Microdevices

, Volume 13, Issue 1, pp 203–213

3D microfilter device for viable circulating tumor cell (CTC) enrichment from blood

  • Siyang Zheng
  • Henry K. Lin
  • Bo Lu
  • Anthony Williams
  • Ram Datar
  • Richard J. Cote
  • Yu-Chong Tai
Article

DOI: 10.1007/s10544-010-9485-3

Cite this article as:
Zheng, S., Lin, H.K., Lu, B. et al. Biomed Microdevices (2011) 13: 203. doi:10.1007/s10544-010-9485-3

Abstract

Detection of circulating tumor cells has emerged as a promising minimally invasive diagnostic and prognostic tool for patients with metastatic cancers. We report a novel three dimensional microfilter device that can enrich viable circulating tumor cells from blood. This device consists of two layers of parylene membrane with pores and gap precisely defined with photolithography. The positions of the pores are shifted between the top and bottom membranes. The bottom membrane supports captured cells and minimize the stress concentration on cell membrane and sustain cell viability during filtration. Viable cell capture on device was investigated with scanning electron microscopy, confocal microscopy, and immunofluorescent staining using model systems of cultured tumor cells spiked in blood or saline. The paper presents and validates this new 3D microfiltration concept for circulation tumor cell enrichment application. The device provides a highly valuable tool for assessing and characterizing viable enriched circulating tumor cells in both research and clinical settings.

Keywords

Circulating tumor cell Microfilter Parylene 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Siyang Zheng
    • 1
  • Henry K. Lin
    • 2
  • Bo Lu
    • 3
  • Anthony Williams
    • 4
  • Ram Datar
    • 4
  • Richard J. Cote
    • 4
  • Yu-Chong Tai
    • 3
  1. 1.Department of BioengineeringPennsylvania State UniversityUniversity ParkUSA
  2. 2.BioSciences DivisionOak Ridge National LabOak RidgeUSA
  3. 3.Department of Electrical EngineeringCalifornia Institute of TechnologyPasadenaUSA
  4. 4.Department of PathologyUniversity of MiamiMiamiUSA

Personalised recommendations