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Calcium-dependent signaling pathway in the heat-induced oxidative injury in Amaranthus lividus

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Biologia Plantarum

Abstract

Heat caused reduction in membrane protein thiol content, increased accumulation of thiobarbituric acid reactive substances and reduced germination rate and early growth in germinating Amaranthus lividus seeds. Imposition of heat stress during early germination also causes accumulation of reactive oxygen species like superoxide and hydrogen peroxide while activities of antioxidative enzymes catalase, ascorbate peroxidase, and glutathione reductase decreased. Calcium chelator (EGTA), calcium channel blocker (LaCl3) and calmodulin inhibitor (trifluroperazine) aggravated these effects. Added calcium reversed the effect of heat, implying that protection against heat induced oxidative damage and improvement of germination requires calcium and calmodulin during the recovery phase of post-germination events in Amaranthus lividus.

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Abbreviations

APOX:

ascorbate peroxidase

CAT:

catalase

DTNB:

5,5-dithio-bis-2-nitrobenzoic acid

GR:

glutathione reductase

HS:

heat stress

RGI:

relative growth index

MPTL:

membrane protein thiol level

RGP:

relative germination performance

ROS:

reactive oxygen species

SOD:

superoxide dismutase

TFP:

trifluroperazine

TBARS:

thiobarbituric acid reactive substances

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Authors and Affiliations

  1. PostgraduateDepartment of Botany, Hooghly Mohsin College, Chinsurah, 712101, West Bengal, India

    S. Bhattacharjee

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  1. S. Bhattacharjee
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Correspondence to S. Bhattacharjee.

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Bhattacharjee, S. Calcium-dependent signaling pathway in the heat-induced oxidative injury in Amaranthus lividus . Biol Plant 52, 137–140 (2008). https://doi.org/10.1007/s10535-008-0028-1

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  • DOI: https://doi.org/10.1007/s10535-008-0028-1

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