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Characterization of the iron-binding properties of pyoverdine using electron-capture dissociation-tandem mass spectrometry

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Abstract

Pyoverdines (PVD) are a group of siderophores produced by fluorescent Pseudomonads. Identification of PVD variants mostly relies on liquid chromatography-tandem mass spectrometry (LC–MS/MS) using collision-induced dissociation (CID). Here, both CID and the novel dissociation technique electron-capture dissociation (ECD) were applied to characterize PVD succinamide and its Fe(III)-chelated complex. The results clearly showed that ECD produced diagnostic side chain fragmentation of the PVD peptide chain and preserved the labile Fe(III) binding to the chromophore in contrast to CID. The ECD technique is therefore expected to support the understanding of strain-specific Fe(III) transport processes of PVDs.

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Acknowledgments

DAV acknowledges general research support by the Alfried Krupp von Bohlen und Halbach-Stiftung. This work was supported in part by the DFG FTICR-MS Facility, INST 256/356-1.

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Authors and Affiliations

  1. Institute of Bioanalytical Chemistry, Saarland University, 66123, Saarbrücken, Germany

    Yulin Qi & Dietrich A. Volmer

  2. Institute of Inorganic and Analytical Chemistry, University of Münster, Münster, Germany

    Heiko Hayen

Authors
  1. Yulin Qi
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  2. Heiko Hayen
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  3. Dietrich A. Volmer
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Correspondence to Dietrich A. Volmer.

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Qi, Y., Hayen, H. & Volmer, D.A. Characterization of the iron-binding properties of pyoverdine using electron-capture dissociation-tandem mass spectrometry. Biometals 29, 53–60 (2016). https://doi.org/10.1007/s10534-015-9895-z

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  • DOI: https://doi.org/10.1007/s10534-015-9895-z

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