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Yersinia ironomics: comparison of iron transporters among Yersiniapestis biotypes and its nearest neighbor, Yersinia pseudotuberculosis

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Abstract

Although Yersinia pestis epidemic biovars and Yersinia pseudotuberculosis are recently diverged, highly related species, they cause different diseases via disparate transmission routes. Since iron transport systems are important for iron acquisition from hosts and for survival in the environment, we have analyzed potential iron transport systems encoded by epidemic and non-epidemic or endemic strains of Y. pestis as well as two virulent Y. pseudotuberculosis strains. Computational biology analysis of these genomes showed a high degree of identity/similarity among 16 proven or possible iron/heme transporters identified. Of these, 7 systems were essentially the same in all seven genomes analyzed. The remaining 9 loci had 2–6 genetic variations among these genomes. Two untested, potential siderophore-dependent systems appear intact in Y. pseudotuberculosis but are disrupted or absent in all the endemic Y. pestis strains as well as the epidemic strains from the antiqua and mediaevalis biovars. Only one of these two loci are obviously disrupted in Y. pestis CO92 (epidemic orientalis biovar). Experimental studies failed to identify a role for hemin uptake systems in the virulence of pneumonic plague and suggest that Y. pestis CO92 does not make a siderophore other than Ybt.

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Acknowledgments

This study was supported by the Department of Homeland Security, Office of Research and Development through Interagency Agreement HSHQDE-05-00317 and Public Health Service grant AI25098. We thank Ildefonso Mier, Jr., Alex Bobrov, and Olga Kirillina for thoughtful discussions and assistance with some experiments. We also thank Scott Bearden for providing Y. pestis strains CO99-3015 and CO99-3015P.

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  1. Stanislav Forman

    Present address: Zymo Research Corporation, Orange, CA, USA

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  1. Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY, USA

    Stanislav Forman, James T. Paulley, Jacqueline D. Fetherston & Robert D. Perry

  2. Department of Biological Sciences, and Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee, Milwaukee, WI, USA

    Yi-Qiang Cheng

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Correspondence to Robert D. Perry.

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S. Forman and J.T. Paulley contributed equally to this study and are co-first authors.

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Forman, S., Paulley, J.T., Fetherston, J.D. et al. Yersinia ironomics: comparison of iron transporters among Yersiniapestis biotypes and its nearest neighbor, Yersinia pseudotuberculosis. Biometals 23, 275–294 (2010). https://doi.org/10.1007/s10534-009-9286-4

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