Skip to main content
SpringerLink
Log in

Hepatoprotective effect of a protein-enriched fraction from the maggots (Musca domestica) against CCl4-induced hepatic damage in rats

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

The hepatoprotective potential of a protein-enriched fraction (PEF) isolated from the maggots of housefly (Musca domestica) was evaluated in rats against carbon tetrachloride (CCl4)-induced acute hepatic damage. Activities of serum aspartate aminotransferase and alanine aminotransferase increased by 4- and 13-fold induced by CCl4, were significantly inhibited by pretreatment with 50, 100 and 200 mg PEF/kg. The formation of malondialdehyde was also significantly decreased in PEF-treated group compared with CCl4-treated group. The treatments with PEF also elevated total protein levels significantly. These results were further supplemented by histopathological examination of liver sections. Hyperplasia of kupffer cells was observed after treatment with PEF (100 and 200 mg/kg). We conclude that PEF is effective in this model of liver damage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Arii S, Imamura M (2000) Physiological role of sinusoidal endothelial cells and Kupffer cells and their implication in the pathogenesis of liver injury. J Hepatobiliary Pancreat Surg 7:40–48

    Article  PubMed  CAS  Google Scholar 

  • Bhandarkar MR, Khan A (2004) Antihepatotoxic effect of Nymphaea stellata willd., against carbon tetrachloride-induced hepatic damage in albino rats. J Ethnopharmacol 91:61–64

    Article  PubMed  Google Scholar 

  • Jeon TI, Hwang SG, Lim BO, Park DK (2003) Extracts of Phellinus linteus grown on germinated brown rice suppress liver damage induced by carbon tetrachloride in rats. Biotechnol Lett 25:2093–2096

    Article  PubMed  CAS  Google Scholar 

  • Lee KJ, Woo ER, Choi CY, Shin DW, Lee DG, You HJ, Jeong HG (2004) Protective effect of acteoside on carbon tetrachloride-induced hepatotoxicity. Life Sci 74:1051–1064

    Article  PubMed  CAS  Google Scholar 

  • Li SZ (1981) Compendium of Materia Medica. People’s Medical Press, Beijing, China, pp 2289–2290

    Google Scholar 

  • Liu JY, Chen CC, Wang WH, Hsu JD, Yang MY, Wang CJ (2006) The protective effects of Hibiscus sabdariffa extract on CCl4-induced liver fibrosis in rats. Food Chem Toxicol 44:336–343

    Article  PubMed  CAS  Google Scholar 

  • Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358

    Article  PubMed  CAS  Google Scholar 

  • Pagliara P, Carlà EC, Caforio S, Chionna A, Massa S, Abbro L, Dini L (2003) Kupffer cells promote lead nitrate-induced hepatocyte apoptosis via oxidative stress. Comp Hepatol 2(1):8

    Article  PubMed  Google Scholar 

  • Sato Y, Yoneda M, Nakamura K, Makino I, Terano A (2003) Protective effect of central thyrotropin-releasing hormone on carbon tetrachloride-induced acute hepatocellular necrosis in rats. J Hepatol 39:47–54

    Article  PubMed  CAS  Google Scholar 

  • Sherman RA, Hall MJ, Thomas S (2000) Medicinal maggots: an ancient remedy for some contemporary afflictions. Annu Rev Entomol 45:55–81

    Article  PubMed  CAS  Google Scholar 

  • Tang X, Gao J, Wang Y, Fan YM, Xu LZ, Zhao XN, Xu Q, Qian ZM (2006) Effective protection of Terminalia catappa L. leaves from damage induced by carbon tetrachloride in liver mitochondria. J Nutr Biochem 17:177–182

    Article  PubMed  CAS  Google Scholar 

  • Tietze F (1969) Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem 27:502–522

    Article  PubMed  CAS  Google Scholar 

  • Wang FR, Huang W, Wang YL, Lei CL (2005) Proteins isolated from the larva of the housefly and their activities on scavenging the hydroxyl radical. Chinese Bull Entomol 42:546–549

    CAS  Google Scholar 

  • Wills PJ, Asha VV (2006) Protective effect of Lygodium flexuosum (L.) Sw. extract against carbon tetrachloride-induced acute liver injury in rats. J Ethnopharmacol 108:320–326

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We would like to thank Professor Shi-Qi Zhou (College of Animal Medicinal Science, Huazhong Agricultural University) and Wen-Xiang Yang (Hubei Provincial Center for Diseases Control and Prevention) for their technical assistance.

Author information

Authors and Affiliations

  1. Institute of Insect Resources, Huazhong Agricultural University, Wuhan, 430070, P.R. China

    Fu-Rong Wang, Hui Ai, Xiao-Min Chen & Chao-Liang Lei

  2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P.R. China

    Chao-Liang Lei

Authors
  1. Fu-Rong Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hui Ai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiao-Min Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chao-Liang Lei
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chao-Liang Lei.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, FR., Ai, H., Chen, XM. et al. Hepatoprotective effect of a protein-enriched fraction from the maggots (Musca domestica) against CCl4-induced hepatic damage in rats. Biotechnol Lett 29, 853–858 (2007). https://doi.org/10.1007/s10529-007-9321-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-007-9321-x

Keywords

Search

Navigation