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Cellular aggregate size as the critical factor for flavonoid production by suspension cultures of Saussurea medusa

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Abstract

Three previously established cell lines (yellow, red and white) of Saussurea medusa were investigated for jaceosidin and hispidulin production. Maximum yields of the jaceosidin and hispidulin were obtained in the red cell line at 75 ± 0.41 and 6.4 ± 0.31 mg l-1. Production of jaceosidin and hispidulin correlated with the sizes of compact callus aggregates (CCA) and cellular viability. In the red cell line, the sizes of CCA were predominantly of 2–4mm diameter and accounted for 64% biomass. This line had a sustained cell viability over 10 successive sub-cultures.

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Author notes

  1. Yan Huang

    Present address: School of Chemical Engineering, Oklahoma State University, Stillwater, OK, USA, 74075

Authors and Affiliations

  1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, P.R. China, 100093

    Chun-xiang Fu, De-xiu Zhao, Yan Huang & Feng-shan Ma

  2. Department of Biology, University of Waterloo, Ontario, Waterloo, Canada, N2L 3G1

    Feng-shan Ma

Authors
  1. Chun-xiang Fu
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  2. De-xiu Zhao
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  3. Yan Huang
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  4. Feng-shan Ma
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Corresponding author

Correspondence to De-xiu Zhao.

Additional information

Revisions requested 3 September 2004/27 October 2004; Revisions received 22 October 2004/18 November 2004

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Fu, Cx., Zhao, Dx., Huang, Y. et al. Cellular aggregate size as the critical factor for flavonoid production by suspension cultures of Saussurea medusa . Biotechnol Lett 27, 91–95 (2005). https://doi.org/10.1007/s10529-004-6934-1

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  • DOI: https://doi.org/10.1007/s10529-004-6934-1

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