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Molecular Variability among Isolates of Fusarium oxysporum Associated with Root Rot Disease of Agave tequilana

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Abstract

In this study, 115 isolates of Fusarium oxysporum from roots of Agave tequilana Weber cv azul plants and soil in commercial plantations in western Mexico were characterized using morphological and molecular methods. Genetic analyses of monosporic isolates included restriction enzyme analysis of rDNA (ARDRA) using HaeIII and HinfI, and genetic diversity was determined using Box-PCR molecular markers. Box-PCR analysis generated 14 groups. The groups correlated highly with the geographic location of the isolate and sample type. These results demonstrate the usefulness of ARDRA and Box-PCR techniques in the molecular characterization of the Fusarium genus for the discrimination of pathogenic isolates.

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Acknowledgments

We would like to thank the DGEST for partial financial assistance. Thanks also to Dr. M. A. Apodaca Sanchez at Universidad Autónoma de Sinaloa for proved FOL and FORL isolates and Mike Davis of the University of California for critical reading of the manuscript. This study forms part of the master’s degree of the first author in UABC.

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Authors and Affiliations

  1. Azul Agricultura y Servicios S.A. de C.V., Circunvalación Sur 51a, 45070, Zapopan, Jalisco, Mexico

    Karla L. Vega-Ramos & J. Xavier Uvalle-Bueno

  2. Laboratorio de Biología Molecular, Instituto Tecnológico de Tlajomulco, Km 10 Carretera a San Miguel Cuyutlán, 45640, Tlajomulco de Zúñiga, Jalisco, Mexico

    Juan F. Gómez-Leyva

Authors
  1. Karla L. Vega-Ramos
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  2. J. Xavier Uvalle-Bueno
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  3. Juan F. Gómez-Leyva
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Correspondence to Juan F. Gómez-Leyva.

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Vega-Ramos, K.L., Uvalle-Bueno, J.X. & Gómez-Leyva, J.F. Molecular Variability among Isolates of Fusarium oxysporum Associated with Root Rot Disease of Agave tequilana . Biochem Genet 51, 243–255 (2013). https://doi.org/10.1007/s10528-012-9559-4

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  • DOI: https://doi.org/10.1007/s10528-012-9559-4

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