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Cloning and Characterization of p8 Homolog cDNA in the Atlantic Halibut (Hippoglossus hippoglossus)

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Abstract

The p8 gene encodes a transcription factor known to modulate cell growth, division, and apoptosis and influences gene expression. In this study, an Atlantic halibut (Hippoglossus hippoglossus) homolog of the p8 gene was cloned, sequenced, and characterized. The full-length p8 cDNA consists of 601 bp and encodes 76 amino acids with a molecular mass of 9 kD. The bHLH region is well conserved between Atlantic halibut and other animals. Analysis by RT-PCR showed that the p8 transcript is constitutively expressed in 9 of the 12 tissues tested: pancreas, intestine, stomach, gill, head kidney, heart, liver, ovary, and spleen. A predicted microRNA target site was found in the 3′UTR of Atlantic halibut p8 mRNA. We speculate that the target site may pair to microRNA molecules because the target site resides in a big loop, a space large enough for the binding of microRNA molecules.

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Acknowledgments

This study was supported by grants from the Research Fund of the Hebei Agriculture of University and the Research Fund of Science and Technology Project of Qin Huang Dao City (Grant No. 200901A068).

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Authors and Affiliations

  1. College of Ocean, Hebei Agriculture University, Hebei Street No. 52, 066003, Qinhuangdao, Hebei, China

    Jia-Qing Wang

  2. Xiamen Ocean Vocational College, 361012, Xiamen, Fujian, China

    Lin-Chun Li

  3. School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, 110016, Shenyang, China

    Sheng-Guo Lin

  4. Wei Nan Teachers University, 714000, Weinan, China

    Zhi-Ping Wang

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  1. Jia-Qing Wang
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  2. Lin-Chun Li
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Correspondence to Jia-Qing Wang.

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Wang, JQ., Li, LC., Lin, SG. et al. Cloning and Characterization of p8 Homolog cDNA in the Atlantic Halibut (Hippoglossus hippoglossus). Biochem Genet 48, 504–515 (2010). https://doi.org/10.1007/s10528-010-9334-3

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  • DOI: https://doi.org/10.1007/s10528-010-9334-3

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