Angiogenesis

, Volume 10, Issue 4, pp 297–305

Caveolin-1 polarization in transmigrating endothelial cells requires binding to intermediate filaments

  • Virginie Santilman
  • JoAnne Baran
  • Bela Anand-Apte
  • Robert M. Evans
  • Marie-Odile Parat
Original Paper

DOI: 10.1007/s10456-007-9083-z

Cite this article as:
Santilman, V., Baran, J., Anand-Apte, B. et al. Angiogenesis (2007) 10: 297. doi:10.1007/s10456-007-9083-z

Abstract

Caveolin-1 influences cell migration through multiple signaling pathways. In a previous report, we have shown that caveolin-1 is polarized in three-dimensional migrating endothelial cells (EC), and that caveolin-1 accumulation at the front of transmigrating cells requires the phosphorylatable Tyr14 residue of caveolin-1. Immuno-electron microscopy further indicated that caveolin-1 was distributed along cytoskeletal structures in the anterior of transmigrating EC [Parat MO, Anand-Apte B, Fox PL (Mol Biol Cell 14:3156–3168, 2003)]. In the present study, we investigate whether caveolin-1 interacts with intermediate filaments (IF) and whether this interaction is required for caveolin-1 polarization in transmigrating cells. The distribution of vimentin is polarized in cells traversing a filter pore and overlaps with the distribution of caveolin-1, which accumulates in the cell front. In vivo sprouting EC also exhibit an anterior polarization of these two proteins. Furthermore, caveolin-1 co-purifies with intermediate filaments, suggesting an interaction between caveolin-1 and IF. Vimentin-deficient SW13 cells exhibit a dramatically altered polarization of caveolin-1-GFP, which no longer accumulates in the protruding cell extension. In addition, the Tyr14 residue of caveolin-1 is required for co-purification of the protein with IF. Taken together, our results show that caveolin-1 Tyr14 is necessary for binding to intermediate filaments, which in turn is required for anterior polarization of caveolin-1 in transmigrating cells.

Keywords

Endothelial cellsVimentinCell motilityMigrationAngiogenesisSprouting

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Virginie Santilman
    • 1
  • JoAnne Baran
    • 1
  • Bela Anand-Apte
    • 2
  • Robert M. Evans
    • 3
  • Marie-Odile Parat
    • 1
    • 4
    • 5
  1. 1.Department of Anesthesiology ResearchCleveland ClinicClevelandUSA
  2. 2.Department of Ophthalmic ResearchCleveland ClinicClevelandUSA
  3. 3.Department of PathologyUniversity of Colorado Health Sciences CenterDenverUSA
  4. 4.Department of Outcomes ResearchCleveland ClinicClevelandUSA
  5. 5.School of PharmacyUniversity of QueenslandSt. LuciaAustralia