Abstract
Purpose
To assess the reproducibility of the new spectral domain Cirrus high-definition optical coherence tomography (HD-OCT; Carl Zeiss Meditec, Dublin, CA, USA) for analysis of peripapillary retinal nerve fiber layer (RNFL) thickness in healthy eyes.
Methods
Thirty healthy Korean volunteers were enrolled. Three optic disc cube 200 × 200 Cirrus HD-OCT scans were taken on the same day in discontinuous sessions by the same operator without using the repeat scan function. The reproducibility of the calculated RNFL thickness and probability code were determined by the intraclass correlation coefficient (ICC), coefficient of variation (CV), test-retest variability, and Fleiss’ generalized kappa (κ).
Results
Thirty-six eyes were analyzed. For average RNFL thickness, the ICC was 0.970, CV was 2.38%, and test-retest variability was 4.5 μm. For all quadrants except the nasal, ICCs were 0.972 or higher and CVs were 4.26% or less. Overall test-retest variability ranged from 5.8 to 8.1 μm. The κ value of probability codes for average RNFL thickness was 0.690. The κ values of quadrants and clock-hour sectors were lower in the nasal areas than in other areas.
Conclusions
The reproducibility of Cirrus HD-OCT to analyze peripapillary RNFL thickness in healthy eyes was excellent compared with the previous reports for time domain Stratus OCT. For the calculated RNFL thickness and probability code, variability was relatively higher in the nasal area, and more careful analyses are needed.
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References
Huang D, Swanson EA, Lin CP, et al. Optical coherence tomography. Science 1991;254:1178–1181.
Hee MR, Izatt JA, Swanson EA, et al. Optical coherence tomography of the human retina. Arch Ophthalmol 1995;113:325–332.
Jaffe GJ, Caprioli J. Optical coherence tomography to detect and manage retinal disease and glaucoma. Am J Ophthalmol 2004;137:156–169.
Greenfield DS, Weinreb RN. Role of optic nerve imaging in glaucoma clinical practice and clinical trials. Am J Ophthalmol 2008;145:598–603.
Nassif N, Cense B, Park BH, et al. In vivo human retinal imaging by ultrahigh-speed spectral domain optical coherence tomography. Opt Lett 2004;29:480–482.
Wojtkowski M, Bajraszewski T, Gorczyńska I, et al. Ophthalmic imaging by spectral optical coherence tomography. Am J Ophthalmol 2004;138:412–419.
Chen TC, Cense B, Pierce MC, et al. Spectral domain optical coherence tomography: ultra-high speed, ultra-high resolution ophthalmic imaging. Arch Ophthalmol 2005;123:1715–1720.
Alasil T, Tan O, Lu AT, Huang D, Sadun AA. Correlation of Fourier domain optical coherence tomography retinal nerve fiber layer maps with visual fields in nonarteritic ischemic optic neuropathy. Ophthalmic Surg Lasers Imaging 2008;39:S71–S79.
Menke MN, Knecht P, Sturm V, Dabov S, Funk J. Reproducibility of nerve fiber layer thickness measurements using 3D Fourierdomain OCT. Invest Ophthalmol Vis Sci 2008;49:5386–5391.
Giraudeau B, Mary JY. Planning a reproducibility study: how many subjects and how many replicates per subject for an expected width of the 95 per cent confidence interval of the intraclass correlation coefficient. Stat Med 2001;20:3205–3214.
Budenz DL, Chang RT, Huang X, Knighton RW, Tielsch JM. Reproducibility of retinal nerve fiber thickness measurements using the stratus OCT in normal and glaucomatous eyes. Invest Ophthalmol Vis Sci 2005;46:2440–2443.
Budenz DL, Fredette MJ, Feuer WJ, Anderson DR. Reproducibility of peripapillary retinal nerve fiber thickness measurements with stratus OCT in glaucomatous eyes. Ophthalmology 2008;115:661–666.
Fleiss JL. Measuring nominal scale agreement among many raters. Psychol Bull 1971;76:378–382.
Fleiss JL, Nee JC, Landis JR. Large sample variance of kappa in the case of different sets of raters. Psychol Bull 1979;86:974–977.
King JE. Software solutions for obtaining a kappa-type statistics for use with multiple raters. Paper presented at the Annual Meeting of the Southwest Educational Research Association. February 2004; Dallas, TX, USA.
Leung CK, Cheung CY, Lin D, Pang CP, Lam DS, Weinreb RN. Longitudinal variability of optic disc and retinal nerve fiber layer measurements. Invest Ophthalmol Vis Sci 2008;49:4886–4892.
Z136 Committee. American national standard for safe use of lasers: ANSI Z136.1-2000. New York: Laser Institute of America; 2007.
Giangiacomo A, Garway-Heath D, Caprioli J. Diagnosing glaucoma progression: current practice and promising technologies. Curr Opin Ophthalmol 2006;17:153–162.
Zangwill LM, Bowd C. Retinal nerve fiber layer analysis in the diagnosis of glaucoma. Curr Opin Ophthalmol 2006;17:120–131.
Hood DC, Kardon RH. A framework for comparing structural and functional measures of glaucomatous damage. Prog Retin Eye Res 2007;26:688–710.
Chang R, Budenz DL. New developments in optical coherence tomography for glaucoma. Curr Opin Ophthalmol 2008;19:127–135.
Lemij HG, Reus NJ. New developments in scanning laser polarimetry for glaucoma. Curr Opin Ophthalmol 2008;19:136–140.
Sim J, Wright CC. The kappa statistic in reliability studies: use, interpretation, and sample size requirements. Phys Ther 2005;85:257–268.
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Hong, S., Kim, C.Y., Lee, W.S. et al. Reproducibility of peripapillary retinal nerve fiber layer thickness with spectral domain cirrus high-definition optical coherence tomography in normal eyes. Jpn J Ophthalmol 54, 43–47 (2010). https://doi.org/10.1007/s10384-009-0762-8
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DOI: https://doi.org/10.1007/s10384-009-0762-8